GRANTA-519
GRANTA-519
规格:
货期:
编号:TS259429
品牌:Testobio
| 产品名称： | GRANTA-519 |
|---|---|
| 商品货号： | TS259429 |
| <strong>Cell line </strong>： | GRANTA-519 |
| DSMZ no. ： | TS259429 |
| Species ： | originally confirmed as human with IEF of AST, LDH, MDH |
| Cell type ： | B cell lymphoma |
| Origin ： | established from the peripheral blood taken in 1991 at relapse of a high-grade B-NHL (leukemic transformation of mantle cell lymphoma, stage IV) diagnosed in a 58-year-old Caucasian woman with previous history of cervical carcinoma. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14490, 14636, 14906, 15850, 15229, 18187 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Commission for Biosafety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used for genetic manipulations. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | spheroid cells growing in suspension, singly or in clumps; image; image; image |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 1.0 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml; split ratio of 1:4 every 3 days |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | about 48 hours |
| Harvest ： | maximum density of about 2.6 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypodiploid karyotype with 8% polyploidy - 44(39-44)<2n>XX, -12, -17, -18, +mar, add(1)(p22), del(3)(p14p23), i(8p), i(8q), add(9)(p22)x1-2, t(11;14)(q13;q32), add(13)(p12), add(18)(q21) - sideline with two copies of der(14) and der(9) - carries t(11;14) and rearrangement at 9p22 associated with CCND1 (cyclin D1) activation and deletion of p15/p16 - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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章丽敏
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朱静
18067106081 -
李晴
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李晶晶
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周雪
13372109301
| 产品名称： | GRANTA-519 |
|---|---|
| 商品货号： | TS259429 |
| <strong>Cell line </strong>： | GRANTA-519 |
| DSMZ no. ： | TS259429 |
| Species ： | originally confirmed as human with IEF of AST, LDH, MDH |
| Cell type ： | B cell lymphoma |
| Origin ： | established from the peripheral blood taken in 1991 at relapse of a high-grade B-NHL (leukemic transformation of mantle cell lymphoma, stage IV) diagnosed in a 58-year-old Caucasian woman with previous history of cervical carcinoma. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14490, 14636, 14906, 15850, 15229, 18187 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Commission for Biosafety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used for genetic manipulations. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | spheroid cells growing in suspension, singly or in clumps; image; image; image |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 1.0 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml; split ratio of 1:4 every 3 days |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | about 48 hours |
| Harvest ： | maximum density of about 2.6 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypodiploid karyotype with 8% polyploidy - 44(39-44)<2n>XX, -12, -17, -18, +mar, add(1)(p22), del(3)(p14p23), i(8p), i(8q), add(9)(p22)x1-2, t(11;14)(q13;q32), add(13)(p12), add(18)(q21) - sideline with two copies of der(14) and der(9) - carries t(11;14) and rearrangement at 9p22 associated with CCND1 (cyclin D1) activation and deletion of p15/p16 - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
