TFK-1
TFK-1
规格:
货期:
编号:TS259447
品牌:Testobio
| 产品名称： | TFK-1 |
|---|---|
| 商品货号： | TS259447 |
| <strong>Cell line </strong>： | TFK-1 |
| DSMZ no. ： | TS259447 |
| Species ： | confirmed originally as human with IEF of AST, MDH, NP |
| Cell type ： | bile duct carcinoma |
| Origin ： | established from the surgical specimen extracted from a 63-year-old Japanese man with extrahepatic bile duct carcinoma; described in the literature to express c-erbB-2 protein |
| Reference(s) ： | 14911 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial cells growing as monolayers; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:3 to 1:5 every 2-3 days using trypsin/EDTA (for 10-15 min) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | cell harvest of ca. 10-30 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypertriploid karyotype with 20% polyploidy - 72(64-81)<3n>XY/XYY, +1, +3, +5, +5, +6, -9, +11, -13, -16, -18, +20, +2mar, del(1)(q21), del(1)(p11), i(1q), del(3)(p12p21)x2, add(5)(q35), i(5p)x2, inv(7)(q11q35), del(11)(p11)x1-2, i(15p), i(17p) - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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联系方式
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | TFK-1 |
|---|---|
| 商品货号： | TS259447 |
| <strong>Cell line </strong>： | TFK-1 |
| DSMZ no. ： | TS259447 |
| Species ： | confirmed originally as human with IEF of AST, MDH, NP |
| Cell type ： | bile duct carcinoma |
| Origin ： | established from the surgical specimen extracted from a 63-year-old Japanese man with extrahepatic bile duct carcinoma; described in the literature to express c-erbB-2 protein |
| Reference(s) ： | 14911 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial cells growing as monolayers; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:3 to 1:5 every 2-3 days using trypsin/EDTA (for 10-15 min) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | cell harvest of ca. 10-30 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypertriploid karyotype with 20% polyploidy - 72(64-81)<3n>XY/XYY, +1, +3, +5, +5, +6, -9, +11, -13, -16, -18, +20, +2mar, del(1)(q21), del(1)(p11), i(1q), del(3)(p12p21)x2, add(5)(q35), i(5p)x2, inv(7)(q11q35), del(11)(p11)x1-2, i(15p), i(17p) - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
