SK-MES-1
SK-MES-1
规格:
货期:
编号:TS259451
品牌:Testobio
| 产品名称： | SK-MES-1 |
|---|---|
| 商品货号： | TS259451 |
| <strong>Cell line </strong>： | SK-MES-1 |
| DSMZ no. ： | TS259451 |
| Species ： | confirmed as human with IEF of AST, LDH, NP |
| Cell type ： | lung squamous cell carcinoma |
| Origin ： | established from the pleural effusion of a 65-year-old Caucasian man with lung squamous cell carcinoma in 1970 |
| Reference(s) ： | 15135 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like adherent cells growing as monolayers |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | split confluent culture about 1:3 to 1:5 every 3-4 days using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/80 cm2 + 10 ml medium |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of ca. 15 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hyperdiploid karyotype with 44% polyploidy - 54-60<2n>X, -Y, +1, +1, -2, +4, +5, +7, +7, +11, +13, +19, +22, +5mar, add(X)(p21), i(1q), del(1)(q12)x1-2, add(2)(q37), del(3)(p22p26), add(4)(q32), der(5)t(5;6)(p15;p11), del(5)(q13), add(7)(q11;abr), add(11)(q2)x2, add(13)(p12)x1-2, add(13)(p12), add(15)(p12), der(16)t(8;16)(q12;q23), del(17)(p11), add(19)(p13)x2-3 - sideline with additional der(7;8)(q10;p10), add(22)(q13), der(11)dup(11)(q13q22) - carries 3p-, 5q-, +7, 17p- associated with NSCLC - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV - |
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联系我们CONTACT US
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0574-87917803
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testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
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联系方式
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | SK-MES-1 |
|---|---|
| 商品货号： | TS259451 |
| <strong>Cell line </strong>： | SK-MES-1 |
| DSMZ no. ： | TS259451 |
| Species ： | confirmed as human with IEF of AST, LDH, NP |
| Cell type ： | lung squamous cell carcinoma |
| Origin ： | established from the pleural effusion of a 65-year-old Caucasian man with lung squamous cell carcinoma in 1970 |
| Reference(s) ： | 15135 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like adherent cells growing as monolayers |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | split confluent culture about 1:3 to 1:5 every 3-4 days using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/80 cm2 + 10 ml medium |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of ca. 15 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hyperdiploid karyotype with 44% polyploidy - 54-60<2n>X, -Y, +1, +1, -2, +4, +5, +7, +7, +11, +13, +19, +22, +5mar, add(X)(p21), i(1q), del(1)(q12)x1-2, add(2)(q37), del(3)(p22p26), add(4)(q32), der(5)t(5;6)(p15;p11), del(5)(q13), add(7)(q11;abr), add(11)(q2)x2, add(13)(p12)x1-2, add(13)(p12), add(15)(p12), der(16)t(8;16)(q12;q23), del(17)(p11), add(19)(p13)x2-3 - sideline with additional der(7;8)(q10;p10), add(22)(q13), der(11)dup(11)(q13q22) - carries 3p-, 5q-, +7, 17p- associated with NSCLC - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV - |
