LAT
LAT
规格:
货期:
编号:TS259456
品牌:Testobio
| 产品名称： | LAT |
|---|---|
| 商品货号： | TS259456 |
| <strong>Cell line </strong>： | LAT |
| DSMZ no. ： | TS259456 |
| Species ： | originally confirmed as sheep by species PCR and recently as ovis aries origin by COI DNA barcoding |
| Cell type ： | aortic tissue cells |
| Origin ： | established from the aortic tissue cells of a lamb; cells were described in the literature to be susceptible to Visna Maedi Virus and to be free of bovine viral diarrhoea virus (BVDV) |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | fibroblastoid, growing in monolayers, forming adherent clumps upon reaching confluency |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split near-confluent culture 1:5 every 4-5 days using trypsin/EDTA; seed out at ca. 1.5 x 106 cells/80 cm2; optimal density at 1.0-4.5 x 104 cells/cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | 30-50 hours |
| Harvest ： | cell harvest of ca. 5 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS and 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Cytogenetics ： | ovine diploid karyotype with 27% polyploidy - 54<2n>XY, no consistent abnormality detected |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |
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联系我们CONTACT US
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0574-87917803
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testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
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联系方式
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | LAT |
|---|---|
| 商品货号： | TS259456 |
| <strong>Cell line </strong>： | LAT |
| DSMZ no. ： | TS259456 |
| Species ： | originally confirmed as sheep by species PCR and recently as ovis aries origin by COI DNA barcoding |
| Cell type ： | aortic tissue cells |
| Origin ： | established from the aortic tissue cells of a lamb; cells were described in the literature to be susceptible to Visna Maedi Virus and to be free of bovine viral diarrhoea virus (BVDV) |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | fibroblastoid, growing in monolayers, forming adherent clumps upon reaching confluency |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split near-confluent culture 1:5 every 4-5 days using trypsin/EDTA; seed out at ca. 1.5 x 106 cells/80 cm2; optimal density at 1.0-4.5 x 104 cells/cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | 30-50 hours |
| Harvest ： | cell harvest of ca. 5 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS and 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Cytogenetics ： | ovine diploid karyotype with 27% polyploidy - 54<2n>XY, no consistent abnormality detected |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |
