KELLY
KELLY
规格:
货期:
编号:TS259464
品牌:Testobio
| 产品名称： | KELLY |
|---|---|
| 商品货号： | TS259464 |
| <strong>Cell line </strong>： | KELLY |
| DSMZ no. ： | TS259464 |
| Species ： | confirmed originally as human with IEF of AST, LDH, MDH |
| Cell type ： | neuroblastoma |
| Origin ： | established from a patient with neuroblastoma; ca. 100-fold genomic amplification of N-MYC in der(17)hsr |
| Reference(s) ： | 14858, 14947 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, round to fusiform with polar neuritic processes, growing in mono- and multilayers; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 2-3 x 106 cells/80 cm2; split confluent culture 1:3 to 1:10 every 3-6 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | cell harvest of ca. 25-30 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-diploid karyotype with 20% polyploidy - 46-47<2n>X, -7, -8, +16, +22, +2mar, del(1)(p34), t(2;7)(p12;q11), add(4)(p15.3), add(5)(p15), add(9)(q34), add(10)(q24), add(11)(q24), add(14)(q32), ins(17;2)(q21;p24 hsr)x2 - del(1p34) and 2p24 hsr are typical of late-stage neuroblastoma - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV +, SMRV - |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | KELLY |
|---|---|
| 商品货号： | TS259464 |
| <strong>Cell line </strong>： | KELLY |
| DSMZ no. ： | TS259464 |
| Species ： | confirmed originally as human with IEF of AST, LDH, MDH |
| Cell type ： | neuroblastoma |
| Origin ： | established from a patient with neuroblastoma; ca. 100-fold genomic amplification of N-MYC in der(17)hsr |
| Reference(s) ： | 14858, 14947 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, round to fusiform with polar neuritic processes, growing in mono- and multilayers; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 2-3 x 106 cells/80 cm2; split confluent culture 1:3 to 1:10 every 3-6 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | cell harvest of ca. 25-30 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-diploid karyotype with 20% polyploidy - 46-47<2n>X, -7, -8, +16, +22, +2mar, del(1)(p34), t(2;7)(p12;q11), add(4)(p15.3), add(5)(p15), add(9)(q34), add(10)(q24), add(11)(q24), add(14)(q32), ins(17;2)(q21;p24 hsr)x2 - del(1p34) and 2p24 hsr are typical of late-stage neuroblastoma - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV +, SMRV - |
