SD-1
SD-1
规格:
货期:
编号:TS259481
品牌:Testobio
| 产品名称： | SD-1 |
|---|---|
| 商品货号： | TS259481 |
| <strong>Cell line </strong>： | SD-1 |
| DSMZ no. ： | TS259481 |
| Species ： | confirmed originally as human with IEF of AST, LDH, NP later by STR fingerprinting |
| Cell type ： | peripheral blood B lymphoblastoid cells (from patient with acute lymphoblastic leukemia) |
| Origin ： | established from the peripheral blood of a woman with acute lymphoblastic leukemia (pre B-ALL) at diagnosis; cells were immortalized with EBV; shown at DSMZ by RT-PCR to carry minor breakpoint BCR-ABL1 fusion for which it is a suitable positive control for RT-PCR |
| Reference(s) ： | 14484, 14458 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | ovoid to round cells growing in suspension, singly or in clumps; image; image; image |
| Medium ： | 85-90% RPMI 1640 + 5-10% h.i. FBS |
| Subculture ： | seed out at ca. 0.3 x 106 cells/ml; maintain at 0.3-1.0 x 106 cells/ml; split cells 1:3 to 1:5 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 28-32 hours |
| Harvest ： | reaches maximum density of ca. 1.3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD37 +, cyCD79a +, CD80 +, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa +, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-tetraploid karyotype - 92(88-92)<4n>XXXX, t(9;22)(q34;q11)x2 - carries two balanced Ph translocations - tetraploid derivate of original diploid karyotype |
| Molec. Genetics ： | expression of fusion gene BCR-ABL1 (e1-a2) confirmed by RT-PCR |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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周雪
13372109301
| 产品名称： | SD-1 |
|---|---|
| 商品货号： | TS259481 |
| <strong>Cell line </strong>： | SD-1 |
| DSMZ no. ： | TS259481 |
| Species ： | confirmed originally as human with IEF of AST, LDH, NP later by STR fingerprinting |
| Cell type ： | peripheral blood B lymphoblastoid cells (from patient with acute lymphoblastic leukemia) |
| Origin ： | established from the peripheral blood of a woman with acute lymphoblastic leukemia (pre B-ALL) at diagnosis; cells were immortalized with EBV; shown at DSMZ by RT-PCR to carry minor breakpoint BCR-ABL1 fusion for which it is a suitable positive control for RT-PCR |
| Reference(s) ： | 14484, 14458 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | ovoid to round cells growing in suspension, singly or in clumps; image; image; image |
| Medium ： | 85-90% RPMI 1640 + 5-10% h.i. FBS |
| Subculture ： | seed out at ca. 0.3 x 106 cells/ml; maintain at 0.3-1.0 x 106 cells/ml; split cells 1:3 to 1:5 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 28-32 hours |
| Harvest ： | reaches maximum density of ca. 1.3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD37 +, cyCD79a +, CD80 +, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa +, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-tetraploid karyotype - 92(88-92)<4n>XXXX, t(9;22)(q34;q11)x2 - carries two balanced Ph translocations - tetraploid derivate of original diploid karyotype |
| Molec. Genetics ： | expression of fusion gene BCR-ABL1 (e1-a2) confirmed by RT-PCR |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
