B-16V
B-16V
规格:
货期:
编号:TS259497
品牌:Testobio
| 产品名称： | B-16V |
|---|---|
| 商品货号： | TS259497 |
| <strong>Cell line </strong>： | B-16V |
| DSMZ no. ： | TS259497 |
| Species ： | originally confirmed as mouse with IEF of AST, LDH, NP, and by species PCR and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | melanoma |
| Origin ： | established from a B16 melanoma tumor grown in syngeneic C57BL/6 mice |
| Reference(s) ： | 14996 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial cells growing in monolayers; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1.5-2.0 x 106 cells/80 cm 2; split semi-confluent culture up to 1:10 about every 3 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 20 hours |
| Harvest ： | cell harvest of ca. 35 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine karyotype |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |
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章丽敏
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朱静
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李晴
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李晶晶
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周雪
13372109301
| 产品名称： | B-16V |
|---|---|
| 商品货号： | TS259497 |
| <strong>Cell line </strong>： | B-16V |
| DSMZ no. ： | TS259497 |
| Species ： | originally confirmed as mouse with IEF of AST, LDH, NP, and by species PCR and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | melanoma |
| Origin ： | established from a B16 melanoma tumor grown in syngeneic C57BL/6 mice |
| Reference(s) ： | 14996 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial cells growing in monolayers; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1.5-2.0 x 106 cells/80 cm 2; split semi-confluent culture up to 1:10 about every 3 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 20 hours |
| Harvest ： | cell harvest of ca. 35 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine karyotype |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |
