T-24
T-24
规格:
货期:
编号:TS259498
品牌:Testobio
| 产品名称： | T-24 |
|---|---|
| 商品货号： | TS259498 |
| <strong>Cell line </strong>： | T-24 |
| DSMZ no. ： | TS259498 |
| Species ： | confirmed as human with IEF of AST, LDH, NP |
| Cell type ： | urinary bladder carcinoma |
| Origin ： | established from the primary tumor of an 81-year-old Caucasian woman with urinary bladder carcinoma (transitional cell carcinoma, grade III) in 1970; described in the literature to produce a variety of cytokines (e.g. G-CSF, IL-6 and SCF) and to carry a p53 mutation |
| Reference(s) ： | 14579, 14410, 14409, 15223, 18168 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid adherent cells growing in monolayers; image; image |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:5 twice a week using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | cell harvest of ca. 10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid karyotype with 8% polyploidy - 70-75<3n>XX, -X, +7, -10, -16, -17, -18, +20, +22, +4mar, add(1)(q11), add(1)(p34), del(6)(q21), del(8)(p21), del(9)(p21), del(10)(p11), add(11)(p13), del(13)(q13q14), i(14q), der(15q21q), add(18)(p11) - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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联系我们CONTACT US
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0574-87917803
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testobio@163.com
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浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
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联系方式
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | T-24 |
|---|---|
| 商品货号： | TS259498 |
| <strong>Cell line </strong>： | T-24 |
| DSMZ no. ： | TS259498 |
| Species ： | confirmed as human with IEF of AST, LDH, NP |
| Cell type ： | urinary bladder carcinoma |
| Origin ： | established from the primary tumor of an 81-year-old Caucasian woman with urinary bladder carcinoma (transitional cell carcinoma, grade III) in 1970; described in the literature to produce a variety of cytokines (e.g. G-CSF, IL-6 and SCF) and to carry a p53 mutation |
| Reference(s) ： | 14579, 14410, 14409, 15223, 18168 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid adherent cells growing in monolayers; image; image |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:5 twice a week using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | cell harvest of ca. 10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid karyotype with 8% polyploidy - 70-75<3n>XX, -X, +7, -10, -16, -17, -18, +20, +22, +4mar, add(1)(q11), add(1)(p34), del(6)(q21), del(8)(p21), del(9)(p21), del(10)(p11), add(11)(p13), del(13)(q13q14), i(14q), der(15q21q), add(18)(p11) - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
