EGI-1
EGI-1
规格:
货期:
编号:TS259525
品牌:Testobio
| 产品名称： | EGI-1 |
|---|---|
| 商品货号： | TS259525 |
| <strong>Cell line </strong>： | EGI-1 |
| DSMZ no. ： | TS259525 |
| Species ： | originally confirmed as human with IEF of AST, MDH, NP, PEP B and later by STR profiling |
| Cell type ： | bile duct carcinoma |
| Origin ： | established from the solid tumor of a 52-year-old Caucasian man with advanced stage malignant bile duct carcinoma (patient had not received any chemotherapy prior to cell explantation) in 1984 and three consecutive passages in female nu/nu mice (NMRI); primary tumor histology: large cell adenocarcinoma of low differentiation, metastasizing (ascites in patient); human character of cell line confirmed by karyotype in passage #34 of in vitro culture (1986) |
| Reference(s) ： | 15157 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent polymorphic (epitheloid or dendritric) cells growing in monolayers with multilayer foci; image; image |
| Medium ： | 90% MEM + 10% h.i. FBS + 2x MEM amino acids (both essential and non-essential) + 4 mM L-glutamine + 1 mM sodium pyruvate |
| Subculture ： | seed out at ca. 1-2 x 106 cells/175 cm2; split confluent culture 1:3 to 1:6 every 3-5 days using trypsin/EDTA (wash twice with PBS, more than 20 min trypsin/EDTA treatment might be required on confluent cultures) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 45-50 hours |
| Harvest ： | cell harvest of ca. 10-12 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid with 8% polyploidy - 70(68-72)<3n>XYY, -8, -10, +11, -12, -15, +21, +5mar, del(4)(q22), i(5q), i(6p)x2, add(18)(q23)x2, del(21)(q22) - rearrangements of 18q and 21q implicated in gall bladder carcinoma |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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testobio@163.com
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联系方式
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | EGI-1 |
|---|---|
| 商品货号： | TS259525 |
| <strong>Cell line </strong>： | EGI-1 |
| DSMZ no. ： | TS259525 |
| Species ： | originally confirmed as human with IEF of AST, MDH, NP, PEP B and later by STR profiling |
| Cell type ： | bile duct carcinoma |
| Origin ： | established from the solid tumor of a 52-year-old Caucasian man with advanced stage malignant bile duct carcinoma (patient had not received any chemotherapy prior to cell explantation) in 1984 and three consecutive passages in female nu/nu mice (NMRI); primary tumor histology: large cell adenocarcinoma of low differentiation, metastasizing (ascites in patient); human character of cell line confirmed by karyotype in passage #34 of in vitro culture (1986) |
| Reference(s) ： | 15157 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent polymorphic (epitheloid or dendritric) cells growing in monolayers with multilayer foci; image; image |
| Medium ： | 90% MEM + 10% h.i. FBS + 2x MEM amino acids (both essential and non-essential) + 4 mM L-glutamine + 1 mM sodium pyruvate |
| Subculture ： | seed out at ca. 1-2 x 106 cells/175 cm2; split confluent culture 1:3 to 1:6 every 3-5 days using trypsin/EDTA (wash twice with PBS, more than 20 min trypsin/EDTA treatment might be required on confluent cultures) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 45-50 hours |
| Harvest ： | cell harvest of ca. 10-12 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid with 8% polyploidy - 70(68-72)<3n>XYY, -8, -10, +11, -12, -15, +21, +5mar, del(4)(q22), i(5q), i(6p)x2, add(18)(q23)x2, del(21)(q22) - rearrangements of 18q and 21q implicated in gall bladder carcinoma |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
