RPMI-8226
RPMI-8226
规格:
货期:
编号:TS259553
品牌:Testobio
| 产品名称： | RPMI-8226 |
|---|---|
| 商品货号： | TS259553 |
| <strong>Cell line </strong>： | RPMI-8226 |
| DSMZ no. ： | TS259553 |
| Species ： | originally confirmed as human with IEF of AST, NP |
| Cell type ： | multiple myeloma |
| Origin ： | established from the peripheral blood of a 61-year-old man with multiple myeloma (IgG lambda-type) at diagnosis in 1966; described in the literature to produce and secrete only lambda light chains (but not heavy chains). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14486, 14749, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | cells grow mostly as single cells in suspension, some cells may grow slightly adherent (occasionally up to 50%); image; image; image ; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.4-0.5 x 106 cells/ml; split saturated culture 1:2 every 3 days; cells may grow slightly adherent, but can be detached by pipetting or with a rubber policeman or with TNE (40 mM TrisCl pH 7.5, 1 mM EDTA pH 7.5, 150 mM NaCl) (trypsin is not necessary) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60-70 hours |
| Harvest ： | cell harvest of ca. 1 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD5 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD38 +, CD80 -, CD138 +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotriploid karyotype with 7.5% polyploidy - 62-67<3n>XXY, -1, +5, +6, +7, -8, -9, -10, +11, -12, -13, -14, +16, +18, -19, +21, -22, der(1)t(1;1)(p36.3;q11-12), add(3)(q27-29)x2, del(4)(p14p15.4), der(5;6)(q10;p10)x1-2, del(5)(q32), del(6)(q15), add(9)(p24), add(11)(p15), del(11)(q25), der(14)t(1;14)(q11-12;q32)x2, del(15)(q11.2q14)x2, add(16)(q23-24), der(17)t(?8;17)(q21.2;q25), del(22)(q13.2) - der(1), 6q-, 14q+ and 22q- associated with multiple myeloma - identified by disomic 14q+ |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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| 产品名称： | RPMI-8226 |
|---|---|
| 商品货号： | TS259553 |
| <strong>Cell line </strong>： | RPMI-8226 |
| DSMZ no. ： | TS259553 |
| Species ： | originally confirmed as human with IEF of AST, NP |
| Cell type ： | multiple myeloma |
| Origin ： | established from the peripheral blood of a 61-year-old man with multiple myeloma (IgG lambda-type) at diagnosis in 1966; described in the literature to produce and secrete only lambda light chains (but not heavy chains). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14486, 14749, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | cells grow mostly as single cells in suspension, some cells may grow slightly adherent (occasionally up to 50%); image; image; image ; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.4-0.5 x 106 cells/ml; split saturated culture 1:2 every 3 days; cells may grow slightly adherent, but can be detached by pipetting or with a rubber policeman or with TNE (40 mM TrisCl pH 7.5, 1 mM EDTA pH 7.5, 150 mM NaCl) (trypsin is not necessary) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60-70 hours |
| Harvest ： | cell harvest of ca. 1 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD5 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD38 +, CD80 -, CD138 +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotriploid karyotype with 7.5% polyploidy - 62-67<3n>XXY, -1, +5, +6, +7, -8, -9, -10, +11, -12, -13, -14, +16, +18, -19, +21, -22, der(1)t(1;1)(p36.3;q11-12), add(3)(q27-29)x2, del(4)(p14p15.4), der(5;6)(q10;p10)x1-2, del(5)(q32), del(6)(q15), add(9)(p24), add(11)(p15), del(11)(q25), der(14)t(1;14)(q11-12;q32)x2, del(15)(q11.2q14)x2, add(16)(q23-24), der(17)t(?8;17)(q21.2;q25), del(22)(q13.2) - der(1), 6q-, 14q+ and 22q- associated with multiple myeloma - identified by disomic 14q+ |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
