VM-CUB1
VM-CUB1
规格:
货期:
编号:TS259555
品牌:Testobio
| 产品名称： | VM-CUB1 |
|---|---|
| 商品货号： | TS259555 |
| <strong>Cell line </strong>： | VM-CUB1 |
| DSMZ no. ： | TS259555 |
| Species ： | originally confirmed as human with IEF of AST, NP |
| Cell type ： | urinary bladder transitional cell carcinoma |
| Origin ： | established from the primary transitional cell carcinoma of the urinary bladder of a male patient; described in the literature to carry a p53 point mutation |
| Reference(s) ： | 14579, 15063 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial-like cells growing as monolayers; image |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:5 to 1:10 every 3-5 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 30-45 hours |
| Harvest ： | cell harvest of ca. 40 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid karyotype with 4% polyploidy - 80<3n>XX/XX?Y, +1, +1, +5, +6, +7, +9, -11, +15, -18, +19, +20, +22, +22, +7mar, del(1)(q21), del(1)(q11), der(1;6)(p10;q10)x2, add(3)(p21.3)x2, i(5p), der(6)t(1;6)(q11;q22), ?inv(7)(p15q35), i(9q), del(10)(p11), del(10)(q?22?24), add(12)(p11)x2, add(15)(p11), add(21)(p11) - sideline with i(5q) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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联系我们CONTACT US
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0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | VM-CUB1 |
|---|---|
| 商品货号： | TS259555 |
| <strong>Cell line </strong>： | VM-CUB1 |
| DSMZ no. ： | TS259555 |
| Species ： | originally confirmed as human with IEF of AST, NP |
| Cell type ： | urinary bladder transitional cell carcinoma |
| Origin ： | established from the primary transitional cell carcinoma of the urinary bladder of a male patient; described in the literature to carry a p53 point mutation |
| Reference(s) ： | 14579, 15063 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial-like cells growing as monolayers; image |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:5 to 1:10 every 3-5 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 30-45 hours |
| Harvest ： | cell harvest of ca. 40 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid karyotype with 4% polyploidy - 80<3n>XX/XX?Y, +1, +1, +5, +6, +7, +9, -11, +15, -18, +19, +20, +22, +22, +7mar, del(1)(q21), del(1)(q11), der(1;6)(p10;q10)x2, add(3)(p21.3)x2, i(5p), der(6)t(1;6)(q11;q22), ?inv(7)(p15q35), i(9q), del(10)(p11), del(10)(q?22?24), add(12)(p11)x2, add(15)(p11), add(21)(p11) - sideline with i(5q) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
