32D
32D
规格:
货期:
编号:TS259571
品牌:Testobio
| 产品名称： | 32D |
|---|---|
| 商品货号： | TS259571 |
| <strong>Cell line </strong>： | 32D |
| DSMZ no. ： | TS259571 |
| Species ： | originally confirmed as mouse with IEF of LDH, NP and with species PCR and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | bone marrow |
| Origin ： | established from long-term bone marrow cultures of C3H/HeJ mice infected with the Friend murine leukemia virus; cells are constitutively growth factor-dependent |
| Reference(s) ： | 14575, 14763, 15034 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | mostly round cells growing singly or in small clusters in suspension, some cells are loosely adherent; image1; image2; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS + 10-20% vol conditioned medium of cell line WEHI-3B (DSM ACC 26) |
| Subculture ： | maintain at 0.02-1.5 x 106 cells/ml; split 1:10 to 1:50 every 2-3 days; seed out at ca. 0.5 x 106 cells/ml. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 15 hours |
| Harvest ： | maximum density at ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD11b +, F4/80 (+); image |
| Cytogenetics ： | murine hypodiploid karyotype - 35(34-35)<2n> - carries 5 centric fusion markers |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |
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周雪
13372109301
| 产品名称： | 32D |
|---|---|
| 商品货号： | TS259571 |
| <strong>Cell line </strong>： | 32D |
| DSMZ no. ： | TS259571 |
| Species ： | originally confirmed as mouse with IEF of LDH, NP and with species PCR and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | bone marrow |
| Origin ： | established from long-term bone marrow cultures of C3H/HeJ mice infected with the Friend murine leukemia virus; cells are constitutively growth factor-dependent |
| Reference(s) ： | 14575, 14763, 15034 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | mostly round cells growing singly or in small clusters in suspension, some cells are loosely adherent; image1; image2; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS + 10-20% vol conditioned medium of cell line WEHI-3B (DSM ACC 26) |
| Subculture ： | maintain at 0.02-1.5 x 106 cells/ml; split 1:10 to 1:50 every 2-3 days; seed out at ca. 0.5 x 106 cells/ml. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 15 hours |
| Harvest ： | maximum density at ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD11b +, F4/80 (+); image |
| Cytogenetics ： | murine hypodiploid karyotype - 35(34-35)<2n> - carries 5 centric fusion markers |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |
