RED-4
RED-4
规格:
货期:
编号:TS259588
品牌:Testobio
| 产品名称： | RED-4 |
|---|---|
| 商品货号： | TS259588 |
| <strong>Cell line </strong>： | RED-4 |
| DSMZ no. ： | TS259588 |
| Species ： | originally confirmed as mouse with IEF of LDH, NP and by species PCR and recently as mus musculus by COI DNA barcoding |
| Cell type ： | erythroid leukemia |
| Origin ： | established from a Rauscher murine leukemia virus-induced erythroid leukemia of a DBA/2 mouse; in contrast to RED-1 (DSM ACC 407), cells were described in the literature to be unresponsive to erythropoietin (EPO) or DMSO |
| Reference(s) ： | 14589 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round, single cells growing in suspension |
| Medium ： | 85-90% Dulbeccos MEM + 10-15% h.i. FBS |
| Subculture ： | maintain at 0.5 x 106 cells/ml; split saturated culture at 1:2 to 1:3 every day; seed out at ca. 1.0 x 106 cells/ml; viability immediately after thawing may be rather low (<30%), but cells recover subsequently over the next week and viability will raise over the next few passages (spent medium should be exchanged without discarding cells); cells grow better in 24-well-plates; maximal density at ca. 1.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 24 hours |
| Harvest ： | cell harvest of ca. 1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine near diploid karyotype with 12.5% polyploidy - 39-42<2n>XX, Df(2E2F1) - 1-2 centric fusion markers present - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase positive |
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周雪
13372109301
| 产品名称： | RED-4 |
|---|---|
| 商品货号： | TS259588 |
| <strong>Cell line </strong>： | RED-4 |
| DSMZ no. ： | TS259588 |
| Species ： | originally confirmed as mouse with IEF of LDH, NP and by species PCR and recently as mus musculus by COI DNA barcoding |
| Cell type ： | erythroid leukemia |
| Origin ： | established from a Rauscher murine leukemia virus-induced erythroid leukemia of a DBA/2 mouse; in contrast to RED-1 (DSM ACC 407), cells were described in the literature to be unresponsive to erythropoietin (EPO) or DMSO |
| Reference(s) ： | 14589 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round, single cells growing in suspension |
| Medium ： | 85-90% Dulbeccos MEM + 10-15% h.i. FBS |
| Subculture ： | maintain at 0.5 x 106 cells/ml; split saturated culture at 1:2 to 1:3 every day; seed out at ca. 1.0 x 106 cells/ml; viability immediately after thawing may be rather low (<30%), but cells recover subsequently over the next week and viability will raise over the next few passages (spent medium should be exchanged without discarding cells); cells grow better in 24-well-plates; maximal density at ca. 1.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 24 hours |
| Harvest ： | cell harvest of ca. 1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine near diploid karyotype with 12.5% polyploidy - 39-42<2n>XX, Df(2E2F1) - 1-2 centric fusion markers present - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase positive |
