SW-1710
SW-1710
规格:
货期:
编号:TS259600
品牌:Testobio
| 产品名称： | SW-1710 |
|---|---|
| 商品货号： | TS259600 |
| <strong>Cell line </strong>： | SW-1710 |
| DSMZ no. ： | TS259600 |
| Species ： | originally confirmed as human with IEF of LDH, NP and recently by STR fingerprinting |
| Cell type ： | urinary bladder transitional cell cacrinoma |
| Origin ： | established from the bladder tumor of an 84-year-old Caucasian woman following transurethral tumor resection in 1977 |
| Reference(s) ： | 14700 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like, elongated cells growing adherently as monolayers; image; image |
| Medium ： | 85% Dulbeccos MEM + 15% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 in 10 ml medium; split confluent culture 1:3 to 1:5 every 2-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 25-32 hours |
| Harvest ： | cell harvest of ca. 6-8 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotetraploid karyotype with 8% polyploidy - 77-84<4n>X, -X, -X, -X, -4, -4, -5, -5, +6, +6, -8, -9, -10, -11, -13, -14, -14, -16, -19, +20, +20, +4mar, der(X)t(X;6)(q11-13;q25-q27), del(2)(p12)x2, add(3)(p14-21), der(6)t(X;6)(q11-13;q25-27)x2, der(6)del(6)(p11p12.2)add(6)(q25-27)x2, add(13)(q33-34) - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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SW-1710
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联系我们CONTACT US
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0574-87917803
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testobio@163.com
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浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
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联系方式
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | SW-1710 |
|---|---|
| 商品货号： | TS259600 |
| <strong>Cell line </strong>： | SW-1710 |
| DSMZ no. ： | TS259600 |
| Species ： | originally confirmed as human with IEF of LDH, NP and recently by STR fingerprinting |
| Cell type ： | urinary bladder transitional cell cacrinoma |
| Origin ： | established from the bladder tumor of an 84-year-old Caucasian woman following transurethral tumor resection in 1977 |
| Reference(s) ： | 14700 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like, elongated cells growing adherently as monolayers; image; image |
| Medium ： | 85% Dulbeccos MEM + 15% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 in 10 ml medium; split confluent culture 1:3 to 1:5 every 2-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 25-32 hours |
| Harvest ： | cell harvest of ca. 6-8 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotetraploid karyotype with 8% polyploidy - 77-84<4n>X, -X, -X, -X, -4, -4, -5, -5, +6, +6, -8, -9, -10, -11, -13, -14, -14, -16, -19, +20, +20, +4mar, der(X)t(X;6)(q11-13;q25-q27), del(2)(p12)x2, add(3)(p14-21), der(6)t(X;6)(q11-13;q25-27)x2, der(6)del(6)(p11p12.2)add(6)(q25-27)x2, add(13)(q33-34) - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
