HSB-2
HSB-2
规格:
货期:
编号:TS259618
品牌:Testobio
| 产品名称： | HSB-2 |
|---|---|
| 商品货号： | TS259618 |
| <strong>Cell line </strong>： | HSB-2 |
| DSMZ no. ： | TS259618 |
| Species ： | confirmed as human with IEF of AST, MDH, PEP B |
| Cell type ： | T cell leukemia |
| Origin ： | established in 1966 from the peripheral blood of an 11-year-old boy with lymphosarcoma that had progressed to acute lymphoblastic leukemia (ALL); cell line was established and initially passaged in newborn hamsters; cells were described as carrying the t(1;7)(p34;q34) involving the LCK and TRB (LCK-TCRB) genes and a submicroscopic del(1)(p32) leading to the STIL-TAL1 (SIL-SCL) fusion gene; one of the oldest immature T cell lines, also known in the literature as CCRF-HSB-2 |
| Reference(s) ： | 14345, 14346 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polygonal cells, growing singly or in dense clumps in suspension; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.3-0.4 x 106 cells/ml; maintain at ca. 0.4-1.0 x 106 cells/ml; split saturated culture 1:3 every 2-3 days; maximal density at ca. 1.3 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 25 hours |
| Harvest ： | cell harvest of ca. 0.8 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD2 -, cyCD3 +, smCD3 -, CD4 -, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, TCRalpha/beta -, TCRgamma/delta -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human pseudodiploid karyotype with 4% polyploidy - 46(42-46)<2n>XY, t(1;7)(p34;q35), carries t(1;7) effecting LCK-TRB (LCK-TCRB) fusion, resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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章丽敏
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朱静
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李晴
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李晶晶
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周雪
13372109301
| 产品名称： | HSB-2 |
|---|---|
| 商品货号： | TS259618 |
| <strong>Cell line </strong>： | HSB-2 |
| DSMZ no. ： | TS259618 |
| Species ： | confirmed as human with IEF of AST, MDH, PEP B |
| Cell type ： | T cell leukemia |
| Origin ： | established in 1966 from the peripheral blood of an 11-year-old boy with lymphosarcoma that had progressed to acute lymphoblastic leukemia (ALL); cell line was established and initially passaged in newborn hamsters; cells were described as carrying the t(1;7)(p34;q34) involving the LCK and TRB (LCK-TCRB) genes and a submicroscopic del(1)(p32) leading to the STIL-TAL1 (SIL-SCL) fusion gene; one of the oldest immature T cell lines, also known in the literature as CCRF-HSB-2 |
| Reference(s) ： | 14345, 14346 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polygonal cells, growing singly or in dense clumps in suspension; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.3-0.4 x 106 cells/ml; maintain at ca. 0.4-1.0 x 106 cells/ml; split saturated culture 1:3 every 2-3 days; maximal density at ca. 1.3 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 25 hours |
| Harvest ： | cell harvest of ca. 0.8 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD2 -, cyCD3 +, smCD3 -, CD4 -, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, TCRalpha/beta -, TCRgamma/delta -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human pseudodiploid karyotype with 4% polyploidy - 46(42-46)<2n>XY, t(1;7)(p34;q35), carries t(1;7) effecting LCK-TRB (LCK-TCRB) fusion, resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
