MOLT-13
MOLT-13
规格:
货期:
编号:TS259619
品牌:Testobio
| 产品名称： | MOLT-13 |
|---|---|
| 商品货号： | TS259619 |
| <strong>Cell line </strong>： | MOLT-13 |
| DSMZ no. ： | TS259619 |
| Species ： | confirmed as human with IEF of AST, NP |
| Cell type ： | T cell leukemia |
| Origin ： | established from the bone marrow of a 2-year-old girl with T-ALL (T cell acute lymphoblastic leukemia, FAB L2) at relapse in 1983; MOLT-14 (DSM ACC 437) is a simultaneous sister cell line derived from the same patient sample with similar or identical features |
| Reference(s) ： | 14477 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round cells with processes, growing in suspension; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/ml in 12-well plates; maintain at a minimum of ca. 1 x 106 cells/ml; cells grow slowly, split saturated culture 1:2 every 5-7 days and change medium twice weekly; cells rarely exceed 80% viability |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 80-120 hours |
| Harvest ： | maximal density at ca. 3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD2 -, CD3 +, CD5 +, CD7 +, CD13 -, CD19 -, CD34 +, TCRalpha/beta -, TCRgamma/delta +; image |
| Fingerprint ： | same DNA profile as MOLT-14 using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human pseudodiploid karyotype with 4% polyploidy - 46(44-48)<2n>XX, der(17)t(17;17)(p13;q22.1) - resembles published karyotype - karyotypically identical to MOLT-14 |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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周雪
13372109301
| 产品名称： | MOLT-13 |
|---|---|
| 商品货号： | TS259619 |
| <strong>Cell line </strong>： | MOLT-13 |
| DSMZ no. ： | TS259619 |
| Species ： | confirmed as human with IEF of AST, NP |
| Cell type ： | T cell leukemia |
| Origin ： | established from the bone marrow of a 2-year-old girl with T-ALL (T cell acute lymphoblastic leukemia, FAB L2) at relapse in 1983; MOLT-14 (DSM ACC 437) is a simultaneous sister cell line derived from the same patient sample with similar or identical features |
| Reference(s) ： | 14477 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round cells with processes, growing in suspension; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/ml in 12-well plates; maintain at a minimum of ca. 1 x 106 cells/ml; cells grow slowly, split saturated culture 1:2 every 5-7 days and change medium twice weekly; cells rarely exceed 80% viability |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 80-120 hours |
| Harvest ： | maximal density at ca. 3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD2 -, CD3 +, CD5 +, CD7 +, CD13 -, CD19 -, CD34 +, TCRalpha/beta -, TCRgamma/delta +; image |
| Fingerprint ： | same DNA profile as MOLT-14 using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human pseudodiploid karyotype with 4% polyploidy - 46(44-48)<2n>XX, der(17)t(17;17)(p13;q22.1) - resembles published karyotype - karyotypically identical to MOLT-14 |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
