YT
YT
规格:
货期:
编号:TS259626
品牌:Testobio
| 产品名称： | YT |
|---|---|
| 商品货号： | TS259626 |
| <strong>Cell line </strong>： | YT |
| DSMZ no. ： | TS259626 |
| Species ： | confirmed as human with IEF of AST, LDH, NP |
| Cell type ： | T/NK cell leukemia |
| Origin ： | established from the pericardial fluid of a 15-year-old man with acute lymphoblastic leukemia (ALL) at relapse (with accompanying thymoma) in 1983; cells were described as: (i) showing NK activity and ADCC (antigen-dependent cell-mediated cytotoxicity), (ii) having their T cell receptor (TCR) genes in germline configuration, and (iii) expressing no TCR proteins (also CD3-, CD16-, CD56+, CD57-). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14487, 14647, 15072, 15074, 18187 |
| Biosafety level ： | 2 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were also positive by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2. |
| Permissions and restrictions ： | A, B |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polygonal cells growing singly or in clumps in suspension; image |
| Medium ： | 80% Iscoves MDM + 20% h.i. FBS |
| Subculture ： | split saturated culture 1:2 every second day; start culture in a 24-well-plate and seed out at ca. 0.8-1.5 x 106 cells/ml, initially after thawing the viability may be reduced down to ca. 20-50% during the first 1-2 weeks (during that initial period, cells should not be diluted, medium may be exchanged without centrifugation); in their vigorous growth phase, maintain at 0.2-0.5 x 106 cells/ml; maximal density at ca. 0.6-0.8 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | cell harvest of ca. 0.6 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD2 -, CD3 -, CD4 -, CD5 -, CD6 -, CD7 +, CD8 -, CD13 -, CD19 -, CD25 (+), CD30 +, CD34 -, TCRalpha/beta -, TCRgamma/delta -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded near-tetraploid karyotype with 12% polyploidy - 84-98<4n>XXYY, -6, -9, -15, -19, +6-8mar, der(X)t(X;7)(q25;q21), der(1)dup(1)(q12.2q2?2)t(1;17)(q3?1;q1?2), der(4)t(1;4)(q32.2;q35)x2, der(5)t(X;5)(p1?3;q21)x2, del(6)(p11), der(10;17)(q10;q10), dup(14)(q1?q2?), add(16)(q24), add(17)(q11) - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV -, HTLV-I/II -, MLV -, SMRV - |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
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李晶晶
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周雪
13372109301
| 产品名称： | YT |
|---|---|
| 商品货号： | TS259626 |
| <strong>Cell line </strong>： | YT |
| DSMZ no. ： | TS259626 |
| Species ： | confirmed as human with IEF of AST, LDH, NP |
| Cell type ： | T/NK cell leukemia |
| Origin ： | established from the pericardial fluid of a 15-year-old man with acute lymphoblastic leukemia (ALL) at relapse (with accompanying thymoma) in 1983; cells were described as: (i) showing NK activity and ADCC (antigen-dependent cell-mediated cytotoxicity), (ii) having their T cell receptor (TCR) genes in germline configuration, and (iii) expressing no TCR proteins (also CD3-, CD16-, CD56+, CD57-). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14487, 14647, 15072, 15074, 18187 |
| Biosafety level ： | 2 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were also positive by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2. |
| Permissions and restrictions ： | A, B |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polygonal cells growing singly or in clumps in suspension; image |
| Medium ： | 80% Iscoves MDM + 20% h.i. FBS |
| Subculture ： | split saturated culture 1:2 every second day; start culture in a 24-well-plate and seed out at ca. 0.8-1.5 x 106 cells/ml, initially after thawing the viability may be reduced down to ca. 20-50% during the first 1-2 weeks (during that initial period, cells should not be diluted, medium may be exchanged without centrifugation); in their vigorous growth phase, maintain at 0.2-0.5 x 106 cells/ml; maximal density at ca. 0.6-0.8 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | cell harvest of ca. 0.6 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD2 -, CD3 -, CD4 -, CD5 -, CD6 -, CD7 +, CD8 -, CD13 -, CD19 -, CD25 (+), CD30 +, CD34 -, TCRalpha/beta -, TCRgamma/delta -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded near-tetraploid karyotype with 12% polyploidy - 84-98<4n>XXYY, -6, -9, -15, -19, +6-8mar, der(X)t(X;7)(q25;q21), der(1)dup(1)(q12.2q2?2)t(1;17)(q3?1;q1?2), der(4)t(1;4)(q32.2;q35)x2, der(5)t(X;5)(p1?3;q21)x2, del(6)(p11), der(10;17)(q10;q10), dup(14)(q1?q2?), add(16)(q24), add(17)(q11) - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV -, HTLV-I/II -, MLV -, SMRV - |
