CAL-33
CAL-33
规格:
货期:
编号:TS259641
品牌:Testobio
| 产品名称： | CAL-33 |
|---|---|
| 商品货号： | TS259641 |
| <strong>Cell line </strong>： | CAL-33 |
| DSMZ no. ： | TS259641 |
| Species ： | human (Homo sapiens) |
| Cell type ： | tongue squamous cell carcinoma |
| Origin ： | established from the surgically removed fragment of a tongue lesion from a 69-year-old man with moderately differentiated squamous cell carcinoma of the tongue in 1983 (prior to therapy) |
| Reference(s) ： | 14562 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherent in monolayers; image; image |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 4 x 106 cells/80 cm2; split confluent culture 1:3 every 4-5 days using trypsin/EDTA (for about 10 min) |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | cell harvest of ca. 25 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 4% polyploidy 49(45-49)<2n>XY/XYY, +7, +9, -18, +20, +mar, add(X)(p22), add(3)(p22-24), i(7p), i(8q), add(9)(p24), del(9)(p2?2) - resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV - |
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朱静
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李晴
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李晶晶
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周雪
13372109301
| 产品名称： | CAL-33 |
|---|---|
| 商品货号： | TS259641 |
| <strong>Cell line </strong>： | CAL-33 |
| DSMZ no. ： | TS259641 |
| Species ： | human (Homo sapiens) |
| Cell type ： | tongue squamous cell carcinoma |
| Origin ： | established from the surgically removed fragment of a tongue lesion from a 69-year-old man with moderately differentiated squamous cell carcinoma of the tongue in 1983 (prior to therapy) |
| Reference(s) ： | 14562 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherent in monolayers; image; image |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 4 x 106 cells/80 cm2; split confluent culture 1:3 every 4-5 days using trypsin/EDTA (for about 10 min) |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | cell harvest of ca. 25 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 4% polyploidy 49(45-49)<2n>XY/XYY, +7, +9, -18, +20, +mar, add(X)(p22), add(3)(p22-24), i(7p), i(8q), add(9)(p24), del(9)(p2?2) - resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV - |
