RED-5

规格:

货期:

编号:TS259653

品牌:Testobio

产品名称：	RED-5
商品货号：	TS259653
<strong>Cell line </strong>：	RED-5
DSMZ no. ：	TS259653
Species ：	confirmed as mouse by species PCR and recently as mus musculus by COI DNA barcoding
Cell type ：	erythroid leukemia
Origin ：	established from a Rauscher murine leukemia virus-induced erythroid leukemia of a female DBA/2 mouse; cells were described as being responsive to erythropoietin (EPO) and to form benzidine-positive colonies upon stimulation with EPO or DMSO
Reference(s) ：	14589
Biosafety level ：	1
Permissions and restrictions ：	A
<strong> </strong>：	DSMZ Scientific Data:
Morphology：	round, single cells growing in suspension
Medium ：	80% RPMI 1640 + 20% h.i. FBS
Subculture ：	split saturated culture at 1:2 to 1:3 every two days; seed out at ca. 0.5-1.0 x 10⁶ cells/ml; viability immediately after thawing may be rather low (<30%), but cells recover subsequently over the next week and viability will raise over the next few passages (spent medium should be exchanged without discarding cells); cells grow initially better in 24-well-plates; maximal density at ca. 2.0 x 10⁶ cells/ml
Incubation ：	at 37 °C with 5% CO₂
Doubling time ：	ca. 30 hours
Harvest ：	cell harvest of ca. 2.0 x 10⁶ cells/ml
Storage ：	frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma ：	negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Cytogenetics ：	murine-like karyotype
Viruses ：	PCR: SMRV -

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RED-5

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产品名称：	RED-5
商品货号：	TS259653
<strong>Cell line </strong>：	RED-5
DSMZ no. ：	TS259653
Species ：	confirmed as mouse by species PCR and recently as mus musculus by COI DNA barcoding
Cell type ：	erythroid leukemia
Origin ：	established from a Rauscher murine leukemia virus-induced erythroid leukemia of a female DBA/2 mouse; cells were described as being responsive to erythropoietin (EPO) and to form benzidine-positive colonies upon stimulation with EPO or DMSO
Reference(s) ：	14589
Biosafety level ：	1
Permissions and restrictions ：	A
<strong> </strong>：	DSMZ Scientific Data:
Morphology：	round, single cells growing in suspension
Medium ：	80% RPMI 1640 + 20% h.i. FBS
Subculture ：	split saturated culture at 1:2 to 1:3 every two days; seed out at ca. 0.5-1.0 x 10⁶ cells/ml; viability immediately after thawing may be rather low (<30%), but cells recover subsequently over the next week and viability will raise over the next few passages (spent medium should be exchanged without discarding cells); cells grow initially better in 24-well-plates; maximal density at ca. 2.0 x 10⁶ cells/ml
Incubation ：	at 37 °C with 5% CO₂
Doubling time ：	ca. 30 hours
Harvest ：	cell harvest of ca. 2.0 x 10⁶ cells/ml
Storage ：	frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma ：	negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Cytogenetics ：	murine-like karyotype
Viruses ：	PCR: SMRV -