BEWO
BEWO
规格:
货期:
编号:TS259661
品牌:Testobio
| 产品名称： | BEWO |
|---|---|
| 商品货号： | TS259661 |
| <strong>Cell line </strong>： | BEWO |
| DSMZ no. ： | TS259661 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | choriocarcinoma |
| Origin ： | a human choriocarcinoma sample was taken at autopsy from a cerebral metastasis; cells were initially transplanted to the cheek pouch of a hamster and maintained by serial passage for 8 years prior to growth in culture vessels |
| Reference(s) ： | 14607, 14836, 14837, 14543 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial-like cells growing as monolayer; image; image |
| Medium ： | 85-90% Hams F12 + 10-15% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:4 - 1:6 every 3-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | cell harvest of ca. 20 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | same DNA profile as JEG-3 using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human hypertriploid karyotype with 24% polyploidy - 78(77-80)<3n>XXYY, +1, +2, +5, +5, +6, +8, +12, -13, +14, -18, +19, -20, +21, +4-6mar, +0-2 dmin, add(X)(p21-22), del(X)(p11), add(1)(p36), der(1)t(1;1)(p3?6;q2?1), i(13q), add(18)(q2?), der(19)t(9;19)(q13;p13), del(22)(q12) - sideline with del(3)(p12p23) - resembles published karyotype and (marginally) that of JEG-3 (DSM ACC 463) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | BEWO |
|---|---|
| 商品货号： | TS259661 |
| <strong>Cell line </strong>： | BEWO |
| DSMZ no. ： | TS259661 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | choriocarcinoma |
| Origin ： | a human choriocarcinoma sample was taken at autopsy from a cerebral metastasis; cells were initially transplanted to the cheek pouch of a hamster and maintained by serial passage for 8 years prior to growth in culture vessels |
| Reference(s) ： | 14607, 14836, 14837, 14543 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial-like cells growing as monolayer; image; image |
| Medium ： | 85-90% Hams F12 + 10-15% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:4 - 1:6 every 3-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | cell harvest of ca. 20 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | same DNA profile as JEG-3 using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human hypertriploid karyotype with 24% polyploidy - 78(77-80)<3n>XXYY, +1, +2, +5, +5, +6, +8, +12, -13, +14, -18, +19, -20, +21, +4-6mar, +0-2 dmin, add(X)(p21-22), del(X)(p11), add(1)(p36), der(1)t(1;1)(p3?6;q2?1), i(13q), add(18)(q2?), der(19)t(9;19)(q13;p13), del(22)(q12) - sideline with del(3)(p12p23) - resembles published karyotype and (marginally) that of JEG-3 (DSM ACC 463) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
