AC-1M46
AC-1M46
规格:
货期:
编号:TS259667
品牌:Testobio
| 产品名称： | AC-1M46 |
|---|---|
| 商品货号： | TS259667 |
| <strong>Cell line </strong>： | AC-1M46 |
| DSMZ no. ： | TS259667 |
| Species ： | human (Homo sapiens) |
| Cell type ： | choriocarcinoma-trophoblast hybrid |
| Origin ： | subclone of ACH1P; ACH1P was established by somatic hybridization of primary male trophoblast cells and the HGPRT-defective choriocarcinoma cell line AC1-1 which was originally derived from cell line JEG-3 (DSM ACC 463) |
| Reference(s) ： | 14543, 15146 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial-like cells growing as monolayer and carrying many intracellular dark granula |
| Medium ： | 90% Hams F12 + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:3 every 3-4 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/80 cm2; maintain at ca. 3 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | cell harvest of ca. 10 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | near identical DNA profiles shared by the AC-sister cell lines, partly identical with JEG-3 and BEWO |
| Cytogenetics ： | human flat-moded hypertetraploid karyotype with 4% polyploidy - 84-107<4n>XXXY, -4, +5, +7, -8, +9, -15, -13, -13, -15, +16, -18, -20, +21, +22, +4-6mar, del(X)(p11p22), der(X)(Xpter->Xq2?8::4p1? ->4p1?::11q1?1->11qter), der(7)t(7;7)(p22;q11.22)add(7)(q32), add(7)(q32), add(9)(p24), add(11)(p15), i(13q), add(15)(p13), add(19)(p13), der(19)t(11;19)(q11;q13) - sideline with: idem, add(8)(q22), +der(7)t(7;7)(p22;q11.22)add(7)(q32) - resembles published karyotype and those of sisters with minor, though consistent, differences (mirroring DNA profiles) - the DNA fingerprint finding of 3 alleles at loci D2S44 and D17S5 suggests additional human chromosomes |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV +, SMRV - |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
AC-1M46
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | AC-1M46 |
|---|---|
| 商品货号： | TS259667 |
| <strong>Cell line </strong>： | AC-1M46 |
| DSMZ no. ： | TS259667 |
| Species ： | human (Homo sapiens) |
| Cell type ： | choriocarcinoma-trophoblast hybrid |
| Origin ： | subclone of ACH1P; ACH1P was established by somatic hybridization of primary male trophoblast cells and the HGPRT-defective choriocarcinoma cell line AC1-1 which was originally derived from cell line JEG-3 (DSM ACC 463) |
| Reference(s) ： | 14543, 15146 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial-like cells growing as monolayer and carrying many intracellular dark granula |
| Medium ： | 90% Hams F12 + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:3 every 3-4 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/80 cm2; maintain at ca. 3 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | cell harvest of ca. 10 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | near identical DNA profiles shared by the AC-sister cell lines, partly identical with JEG-3 and BEWO |
| Cytogenetics ： | human flat-moded hypertetraploid karyotype with 4% polyploidy - 84-107<4n>XXXY, -4, +5, +7, -8, +9, -15, -13, -13, -15, +16, -18, -20, +21, +22, +4-6mar, del(X)(p11p22), der(X)(Xpter->Xq2?8::4p1? ->4p1?::11q1?1->11qter), der(7)t(7;7)(p22;q11.22)add(7)(q32), add(7)(q32), add(9)(p24), add(11)(p15), i(13q), add(15)(p13), add(19)(p13), der(19)t(11;19)(q11;q13) - sideline with: idem, add(8)(q22), +der(7)t(7;7)(p22;q11.22)add(7)(q32) - resembles published karyotype and those of sisters with minor, though consistent, differences (mirroring DNA profiles) - the DNA fingerprint finding of 3 alleles at loci D2S44 and D17S5 suggests additional human chromosomes |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV +, SMRV - |
