GH3
GH3
规格:
货期:
编号:TS259682
品牌:Testobio
| 产品名称： | GH3 |
|---|---|
| 商品货号： | TS259682 |
| <strong>Cell line </strong>： | GH3 |
| DSMZ no. ： | TS259682 |
| Species ： | originally confirmed as rat by cytogenetics and species PCR and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type ： | pituitary tumor |
| Origin ： | derived in 1965 from a pituitary tumor induced in a female Wistar/Furth rat following repeated transplantation with the MtT/W5 tumor (originally induced in a female F/Fu rat by cranial X-irradiation) |
| Reference(s) ： | 15004, 15008 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in loosely adherent in semi-confluent monolayers and as cell clusters in suspension; image |
| Medium ： | 82.5% Hams F10 + 15% horse serum + 2.5% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | split confluent culture 1:2 to 1:5 every 2-5 days, cells may be detached using trypsin/EDTA or mechanically by agitation; seed out at ca. 4 x 106 cells/80 cm2; maintain at 0.3-0.5 x 105 cells/cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60-90 hours |
| Harvest ： | maximal density of 2 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with ciprofloxacin (Ciprobay), then negative in microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | hypertriploid rat karyotype with 20% poylpoidy - 68-71<3n> |
| Viruses ： | PCR: SMRV - |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
GH3
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | GH3 |
|---|---|
| 商品货号： | TS259682 |
| <strong>Cell line </strong>： | GH3 |
| DSMZ no. ： | TS259682 |
| Species ： | originally confirmed as rat by cytogenetics and species PCR and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type ： | pituitary tumor |
| Origin ： | derived in 1965 from a pituitary tumor induced in a female Wistar/Furth rat following repeated transplantation with the MtT/W5 tumor (originally induced in a female F/Fu rat by cranial X-irradiation) |
| Reference(s) ： | 15004, 15008 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in loosely adherent in semi-confluent monolayers and as cell clusters in suspension; image |
| Medium ： | 82.5% Hams F10 + 15% horse serum + 2.5% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | split confluent culture 1:2 to 1:5 every 2-5 days, cells may be detached using trypsin/EDTA or mechanically by agitation; seed out at ca. 4 x 106 cells/80 cm2; maintain at 0.3-0.5 x 105 cells/cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60-90 hours |
| Harvest ： | maximal density of 2 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with ciprofloxacin (Ciprobay), then negative in microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | hypertriploid rat karyotype with 20% poylpoidy - 68-71<3n> |
| Viruses ： | PCR: SMRV - |
