RED-6
RED-6
规格:
货期:
编号:TS259685
品牌:Testobio
| 产品名称： | RED-6 |
|---|---|
| 商品货号： | TS259685 |
| <strong>Cell line </strong>： | RED-6 |
| DSMZ no. ： | TS259685 |
| Species ： | confirmed as mouse by species PCR and recently as mus musculus by COI DNA barcoding |
| Cell type ： | erythroid leukemia |
| Origin ： | established from the liver of a Rauscher murine leukemia virus-induced erythroid leukemia of a female DBA/2 mouse; cells were described as being differentiation-responsive to erythropoietin (EPO) and unresponsive to DSMO |
| Reference(s) ： | 14589 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round single cells growing in suspension, some cells are slightly adherent |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split saturated culture at 1:2 to 1:3 every day; seed out at ca. 0.2-0.5 x 106 cells/ml; viability immediately after thawing may be rather low (<30%), but cells recover subsequently over the next week and viability will raise over the next few passages; maximal density at ca. 3.0 x 106 cells/ml; maintain at ca. 0.5-1.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 18 hours |
| Harvest ： | cell harvest of ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine near-diploid karyotype with 10% polyploidy |
| Viruses ： | PCR: SMRV - |
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周雪
13372109301
| 产品名称： | RED-6 |
|---|---|
| 商品货号： | TS259685 |
| <strong>Cell line </strong>： | RED-6 |
| DSMZ no. ： | TS259685 |
| Species ： | confirmed as mouse by species PCR and recently as mus musculus by COI DNA barcoding |
| Cell type ： | erythroid leukemia |
| Origin ： | established from the liver of a Rauscher murine leukemia virus-induced erythroid leukemia of a female DBA/2 mouse; cells were described as being differentiation-responsive to erythropoietin (EPO) and unresponsive to DSMO |
| Reference(s) ： | 14589 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round single cells growing in suspension, some cells are slightly adherent |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split saturated culture at 1:2 to 1:3 every day; seed out at ca. 0.2-0.5 x 106 cells/ml; viability immediately after thawing may be rather low (<30%), but cells recover subsequently over the next week and viability will raise over the next few passages; maximal density at ca. 3.0 x 106 cells/ml; maintain at ca. 0.5-1.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 18 hours |
| Harvest ： | cell harvest of ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine near-diploid karyotype with 10% polyploidy |
| Viruses ： | PCR: SMRV - |
