CAT-13.9C1
CAT-13.9C1
规格:
货期:
编号:TS259691
品牌:Testobio
| 产品名称： | CAT-13.9C1 |
|---|---|
| 商品货号： | TS259691 |
| <strong>Cell line </strong>： | CAT-13.9C1 |
| DSMZ no. ： | TS259691 |
| Species ： | origin confirmed as mus musculus by COI DNA Barcoding |
| Cell type ： | hybridoma (CD23) |
| Origin ： | described as secreting a mouse monoclonal antibody (IgG2akappa) CD23; the cell line was established in 1988 by fusion of PX63AG8.653 cells with spleen cells of a mouse immunized with an EBV-transformed human B cell line from a patient with MHC class II deficiency re-expressing class II antigens |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells, growing singly or in small clumps |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement" for the first 1-2 weeks post-thawing) |
| Subculture ： | split saturated culture 1:2 every 2 days; seed out at about 0.2 x 106 cells/ml; maintain between 0.2-0.8 x 106 cells/ml |
| Incubation ： | at 37°C with 10% CO2 |
| Doubling time ： | about 48 hours |
| Harvest ： | maximal density of about 1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | secretion of a IgG2a monoclonal antibody |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |
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朱静
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周雪
13372109301
| 产品名称： | CAT-13.9C1 |
|---|---|
| 商品货号： | TS259691 |
| <strong>Cell line </strong>： | CAT-13.9C1 |
| DSMZ no. ： | TS259691 |
| Species ： | origin confirmed as mus musculus by COI DNA Barcoding |
| Cell type ： | hybridoma (CD23) |
| Origin ： | described as secreting a mouse monoclonal antibody (IgG2akappa) CD23; the cell line was established in 1988 by fusion of PX63AG8.653 cells with spleen cells of a mouse immunized with an EBV-transformed human B cell line from a patient with MHC class II deficiency re-expressing class II antigens |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells, growing singly or in small clumps |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement" for the first 1-2 weeks post-thawing) |
| Subculture ： | split saturated culture 1:2 every 2 days; seed out at about 0.2 x 106 cells/ml; maintain between 0.2-0.8 x 106 cells/ml |
| Incubation ： | at 37°C with 10% CO2 |
| Doubling time ： | about 48 hours |
| Harvest ： | maximal density of about 1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | secretion of a IgG2a monoclonal antibody |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |
