CF-10H5
CF-10H5
规格:
货期:
编号:TS259703
品牌:Testobio
| 产品名称： | CF-10H5 |
|---|---|
| 商品货号： | TS259703 |
| <strong>Cell line </strong>： | CF-10H5 |
| DSMZ no. ： | TS259703 |
| Species ： | origin confirmed as mus musculus by COI DNA Barcoding |
| Cell type ： | hybridoma (CD45) |
| Origin ： | described as secreting a mouse monoclonal antibody (IgG1) CD45; the cell line was established in 1989 by fusion of PX63AG8.653 cells with spleen cells of a mouse immunized with IL-2-driven T cell lines from patients with high numbers of gamma-delta T cells in the peripheral blood |
| Reference(s) ： | 15170 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round cells growing in suspension, some loosely adherent |
| Medium ： | 80% Dulbeccos MEM (4.5 g/L glucose) + 20% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement" for the first 1-2 weeks post-thawing) |
| Subculture ： | split saturated culture 1:3 every 1-2 days; seed out at about 0.2 x 106 cells/ml; maintain between 0.2-0.4 x 106 cells/ml; after thawing cells may start slowly to take up growth and may never exceed a viability of 80% |
| Incubation ： | at 37°C with 10% CO2 |
| Doubling time ： | about 30-45 hours |
| Harvest ： | maximal density of about 0.6 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | secretion of a IgG1 monoclonal antibody |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |
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周雪
13372109301
| 产品名称： | CF-10H5 |
|---|---|
| 商品货号： | TS259703 |
| <strong>Cell line </strong>： | CF-10H5 |
| DSMZ no. ： | TS259703 |
| Species ： | origin confirmed as mus musculus by COI DNA Barcoding |
| Cell type ： | hybridoma (CD45) |
| Origin ： | described as secreting a mouse monoclonal antibody (IgG1) CD45; the cell line was established in 1989 by fusion of PX63AG8.653 cells with spleen cells of a mouse immunized with IL-2-driven T cell lines from patients with high numbers of gamma-delta T cells in the peripheral blood |
| Reference(s) ： | 15170 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round cells growing in suspension, some loosely adherent |
| Medium ： | 80% Dulbeccos MEM (4.5 g/L glucose) + 20% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement" for the first 1-2 weeks post-thawing) |
| Subculture ： | split saturated culture 1:3 every 1-2 days; seed out at about 0.2 x 106 cells/ml; maintain between 0.2-0.4 x 106 cells/ml; after thawing cells may start slowly to take up growth and may never exceed a viability of 80% |
| Incubation ： | at 37°C with 10% CO2 |
| Doubling time ： | about 30-45 hours |
| Harvest ： | maximal density of about 0.6 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | secretion of a IgG1 monoclonal antibody |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |
