RH-30
RH-30
规格:
货期:
编号:TS259727
品牌:Testobio
| 产品名称： | RH-30 |
|---|---|
| 商品货号： | TS259727 |
| <strong>Cell line </strong>： | RH-30 |
| DSMZ no. ： | TS259727 |
| Species ： | human (Homo sapiens) |
| Cell type ： | rhabdomyosarcoma |
| Origin ： | established from the bone marrow metastasis of a 17-year-old man with undifferentiated (standard classification) or unclassified (Palmer classification) alveolar rhabdomyosarcoma; cells were described as carrying a p53 mutation, expressing high levels of both myogenin and myoD, and expressing the Pax3/FKHR fusion protein secondary to the t(2;13)(q35;q14) translocation |
| Reference(s) ： | 14467, 15010, 14558 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial-like cells growing as monolayer; image |
| Medium ： | 90-95% RPMI 1640 + 5-10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-1 x 106 cells/80 cm2; split confluent culture 1:5 to 1:10 every 3-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35 hours |
| Harvest ： | cell harvest of ca. 10-15 x 106 cells/80 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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| 产品名称： | RH-30 |
|---|---|
| 商品货号： | TS259727 |
| <strong>Cell line </strong>： | RH-30 |
| DSMZ no. ： | TS259727 |
| Species ： | human (Homo sapiens) |
| Cell type ： | rhabdomyosarcoma |
| Origin ： | established from the bone marrow metastasis of a 17-year-old man with undifferentiated (standard classification) or unclassified (Palmer classification) alveolar rhabdomyosarcoma; cells were described as carrying a p53 mutation, expressing high levels of both myogenin and myoD, and expressing the Pax3/FKHR fusion protein secondary to the t(2;13)(q35;q14) translocation |
| Reference(s) ： | 14467, 15010, 14558 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial-like cells growing as monolayer; image |
| Medium ： | 90-95% RPMI 1640 + 5-10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-1 x 106 cells/80 cm2; split confluent culture 1:5 to 1:10 every 3-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35 hours |
| Harvest ： | cell harvest of ca. 10-15 x 106 cells/80 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
