RH-1
RH-1
规格:
货期:
编号:TS259730
品牌:Testobio
| 产品名称： | RH-1 |
|---|---|
| 商品货号： | TS259730 |
| <strong>Cell line </strong>： | RH-1 |
| DSMZ no. ： | TS259730 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | rhabdomyosarcoma (reclassified as Ewing family tumor based on molecular signature) |
| Origin ： | derived from the bone marrow metastasis of a malignant embryonal rhabdomyosarcoma in a male patient |
| Reference(s) ： | 14894, 15159, 14789 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in monolayers |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:4 to 1:20 every 3-10 days using trypsin/EDTA (short exposure only); seed out at ca. 0.5-1.0 x 106 cells/80 cm2; maximal density at ca. 4 x 105 cells/cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | cell harvest of ca. 1-2 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotriploid karyotype with 12% polyploidy - 67(61-67)<3n>XX, -Y, +2, -3, +7, -8, -10, +12, -14, -15, -16, +19, -22, add(X)(p22), del(1)(p3?5p3?6), del(2)(p14)x1-2, del(2)(q23), del(9)(p11p13), ?add(11)(q25)x2, der(11)t(2;11)(q?3?q?3?)t(2;3)(q3?;p21), add(12)(q13)/dup(12)(q13q21), del(13)(?q21), der(14)t(Y;14)(q11.2;p11)x2, der(16)t(16;22)(q22;q22)x2, del(18)(q21), add(19)(q13), del(22)(q13) - sideline with +7, +21, add(2)(q11), add(13)(p11) - absence of t(2;13) confirmed by FISH |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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章丽敏
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朱静
18067106081 -
李晴
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李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | RH-1 |
|---|---|
| 商品货号： | TS259730 |
| <strong>Cell line </strong>： | RH-1 |
| DSMZ no. ： | TS259730 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | rhabdomyosarcoma (reclassified as Ewing family tumor based on molecular signature) |
| Origin ： | derived from the bone marrow metastasis of a malignant embryonal rhabdomyosarcoma in a male patient |
| Reference(s) ： | 14894, 15159, 14789 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in monolayers |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:4 to 1:20 every 3-10 days using trypsin/EDTA (short exposure only); seed out at ca. 0.5-1.0 x 106 cells/80 cm2; maximal density at ca. 4 x 105 cells/cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | cell harvest of ca. 1-2 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotriploid karyotype with 12% polyploidy - 67(61-67)<3n>XX, -Y, +2, -3, +7, -8, -10, +12, -14, -15, -16, +19, -22, add(X)(p22), del(1)(p3?5p3?6), del(2)(p14)x1-2, del(2)(q23), del(9)(p11p13), ?add(11)(q25)x2, der(11)t(2;11)(q?3?q?3?)t(2;3)(q3?;p21), add(12)(q13)/dup(12)(q13q21), del(13)(?q21), der(14)t(Y;14)(q11.2;p11)x2, der(16)t(16;22)(q22;q22)x2, del(18)(q21), add(19)(q13), del(22)(q13) - sideline with +7, +21, add(2)(q11), add(13)(p11) - absence of t(2;13) confirmed by FISH |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
