RH-18
RH-18
规格:
货期:
编号:TS259737
品牌:Testobio
| 产品名称： | RH-18 |
|---|---|
| 商品货号： | TS259737 |
| <strong>Cell line </strong>： | RH-18 |
| DSMZ no. ： | TS259737 |
| Species ： | originally confirmed as human by cytogenetics |
| Cell type ： | rhabdomyosarcoma |
| Origin ： | cell line was derived from the tumor material xenografted in immuno-deficient mice; tumor material: primary pediatric embryonal rhabdomyosarcoma exhibiting moderate histological differentiation |
| Reference(s) ： | 14603, 14467 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | large transparent epitheloid cells, partially with long extensions, growing as monolayers; image ; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/80cm2; split culture 1:3 every 3-6 days (before reaching confluency) using trypsin/EDTA; confluency of the culture might result in reduced growth rate after passaging |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60-70 hours |
| Harvest ： | maximum cell harvest of ca. 4 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat moded hypotetraploid karyotype with 20% polyploidy - 78-87<4n>XX, -3, -4, -4, +5, +7, +7, -10, -10, -12, -12, -15, -19, +20, +20, +20, -21, +1-2mar, +0-4 dmin, add(1)(p11), del(5)(q13), add(16)(p13), del(16)(p13.2) - sideline with i(3q) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
RH-18
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | RH-18 |
|---|---|
| 商品货号： | TS259737 |
| <strong>Cell line </strong>： | RH-18 |
| DSMZ no. ： | TS259737 |
| Species ： | originally confirmed as human by cytogenetics |
| Cell type ： | rhabdomyosarcoma |
| Origin ： | cell line was derived from the tumor material xenografted in immuno-deficient mice; tumor material: primary pediatric embryonal rhabdomyosarcoma exhibiting moderate histological differentiation |
| Reference(s) ： | 14603, 14467 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | large transparent epitheloid cells, partially with long extensions, growing as monolayers; image ; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/80cm2; split culture 1:3 every 3-6 days (before reaching confluency) using trypsin/EDTA; confluency of the culture might result in reduced growth rate after passaging |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60-70 hours |
| Harvest ： | maximum cell harvest of ca. 4 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat moded hypotetraploid karyotype with 20% polyploidy - 78-87<4n>XX, -3, -4, -4, +5, +7, +7, -10, -10, -12, -12, -15, -19, +20, +20, +20, -21, +1-2mar, +0-4 dmin, add(1)(p11), del(5)(q13), add(16)(p13), del(16)(p13.2) - sideline with i(3q) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
