MEC-2
MEC-2
规格:
货期:
编号:TS259744
品牌:Testobio
| 产品名称： | MEC-2 |
|---|---|
| 商品货号： | TS259744 |
| <strong>Cell line </strong>： | MEC-2 |
| DSMZ no. ： | TS259744 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | chronic B cell leukemia |
| Origin ： | established in 1994 from the peripheral blood of a 62-year-old Caucasian man with chronic B cell leukemia (B-CLL in prolymphocytoid transformation to B-PLL) prior to therapy; serial sister cell line of MEC-1 (DSM ACC 497) |
| Reference(s) ： | 14980 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Commission for Biosafety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used for genetic manipulations. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | polymorphic cells growing in suspension in large clumps; clumps can easily be segregated; image |
| Medium ： | 90% Iscoves MDM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.3-0.5 x 106 cells/ml; maintain at about 0.5 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days; after thawing, during the first week cells should be kept with 20% FBS; starting the culture in 24-well-plate is of advantage; viability may never exceed 80% |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | maximal density at about 1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD80 +, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image |
| Fingerprint ： | same DNA profile as MEC-1 using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human flat-moded hypertriploid karyotype with 7% polyploidy - 75-80<3n>XX/XXY, -6, -9, -10, +11, +12, -13, +15, -17, -21, +10mar - add(X)(p22)x2, inv(12)(?p12.2?q24.1)x2, add(18)(q2?1) - karyotypically distinct from MEC-1 (as reported) |
| Viruses ： | PCR: EBV +, HBV -, HCV-1 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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周雪
13372109301
| 产品名称： | MEC-2 |
|---|---|
| 商品货号： | TS259744 |
| <strong>Cell line </strong>： | MEC-2 |
| DSMZ no. ： | TS259744 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | chronic B cell leukemia |
| Origin ： | established in 1994 from the peripheral blood of a 62-year-old Caucasian man with chronic B cell leukemia (B-CLL in prolymphocytoid transformation to B-PLL) prior to therapy; serial sister cell line of MEC-1 (DSM ACC 497) |
| Reference(s) ： | 14980 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Commission for Biosafety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used for genetic manipulations. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | polymorphic cells growing in suspension in large clumps; clumps can easily be segregated; image |
| Medium ： | 90% Iscoves MDM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.3-0.5 x 106 cells/ml; maintain at about 0.5 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days; after thawing, during the first week cells should be kept with 20% FBS; starting the culture in 24-well-plate is of advantage; viability may never exceed 80% |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | maximal density at about 1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD80 +, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image |
| Fingerprint ： | same DNA profile as MEC-1 using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human flat-moded hypertriploid karyotype with 7% polyploidy - 75-80<3n>XX/XXY, -6, -9, -10, +11, +12, -13, +15, -17, -21, +10mar - add(X)(p22)x2, inv(12)(?p12.2?q24.1)x2, add(18)(q2?1) - karyotypically distinct from MEC-1 (as reported) |
| Viruses ： | PCR: EBV +, HBV -, HCV-1 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
