HCC-1599
HCC-1599
规格:
货期:
编号:TS259753
品牌:Testobio
| 产品名称： | HCC-1599 |
|---|---|
| 商品货号： | TS259753 |
| <strong>Cell line </strong>： | HCC-1599 |
| DSMZ no. ： | TS259753 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | breast carcinoma |
| Origin ： | established in 1995 from the primary tumor cells of a 44-year-old woman with breast carcinoma (ductal type; TNM stage IIIA, grade 3); matched EBV+ B-lymphoblastoid cell line (B-LCL) is available (HCC-1599BL) |
| Reference(s) ： | 14550 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | cells grow as aggregates in suspension; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split saturated culture 1:2 every 4 days; seed out at ca. 3-5 x 106 cells in 5 ml; it might be of advantage to start culture initially in a 6-well-plate, otherwise cells can be grown in flasks; cell numbers can only be estimated as the cells aggregate in large clumps which are rather difficult to disperse; replace half the medium every 2nd day, cells should then be dispersed by pipetting |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 4-5 days |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human highly rearranged flat-moded hypertetraploid karyotype - 92-105<4n>XX, -X, -X, -2, -5, -5, -5, +7, -9, +10, -11, -13, -13, -14, -15, -16, -17, -17, +18, +19, -20, -20, -20, -21, -22, -22, -22, +27-30mar, del(X)(q23), add(1)(p36)x2, der(1;3)(q10;q10)del(1)(p35), del(1)(p35), add(2)(q3?), add(3)(q2?)x2, add(4)(q2?)x2, der(4;14)(q10;q10), add(5)(p15), add(6)(p23), add(6)(q25)x2, add(7)(p11), add(7)(p22)x2, add(7)(q31), add(7)(p11), add(8)(p11), add(8)(q24), add(9)(p22), add(9)(p11), add(10)(q24)x2, add(11)(p12), add(11)(q2?), del(11)(q13), i(13q), add(14)(p11), del(16)(p13.2), add(16)(q22), add(16)(q24), add(19)(q13)x2, add(19)(q13.2), add(21)(p11) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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周雪
13372109301
| 产品名称： | HCC-1599 |
|---|---|
| 商品货号： | TS259753 |
| <strong>Cell line </strong>： | HCC-1599 |
| DSMZ no. ： | TS259753 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | breast carcinoma |
| Origin ： | established in 1995 from the primary tumor cells of a 44-year-old woman with breast carcinoma (ductal type; TNM stage IIIA, grade 3); matched EBV+ B-lymphoblastoid cell line (B-LCL) is available (HCC-1599BL) |
| Reference(s) ： | 14550 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | cells grow as aggregates in suspension; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split saturated culture 1:2 every 4 days; seed out at ca. 3-5 x 106 cells in 5 ml; it might be of advantage to start culture initially in a 6-well-plate, otherwise cells can be grown in flasks; cell numbers can only be estimated as the cells aggregate in large clumps which are rather difficult to disperse; replace half the medium every 2nd day, cells should then be dispersed by pipetting |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 4-5 days |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human highly rearranged flat-moded hypertetraploid karyotype - 92-105<4n>XX, -X, -X, -2, -5, -5, -5, +7, -9, +10, -11, -13, -13, -14, -15, -16, -17, -17, +18, +19, -20, -20, -20, -21, -22, -22, -22, +27-30mar, del(X)(q23), add(1)(p36)x2, der(1;3)(q10;q10)del(1)(p35), del(1)(p35), add(2)(q3?), add(3)(q2?)x2, add(4)(q2?)x2, der(4;14)(q10;q10), add(5)(p15), add(6)(p23), add(6)(q25)x2, add(7)(p11), add(7)(p22)x2, add(7)(q31), add(7)(p11), add(8)(p11), add(8)(q24), add(9)(p22), add(9)(p11), add(10)(q24)x2, add(11)(p12), add(11)(q2?), del(11)(q13), i(13q), add(14)(p11), del(16)(p13.2), add(16)(q22), add(16)(q24), add(19)(q13)x2, add(19)(q13.2), add(21)(p11) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
