MEL-745A cl. DS19
MEL-745A cl. DS19
规格:
货期:
编号:TS259757
品牌:Testobio
| 产品名称： | MEL-745A cl. DS19 |
|---|---|
| 商品货号： | TS259757 |
| <strong>Cell line </strong>： | MEL-745A cl. DS19 |
| DSMZ no. ： | TS259757 |
| Species ： | confirmed as mouse by species PCR and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | erythroleukemia |
| Origin ： | Mouse erythroleukemia (MEL) cells were transformed by Friend leukemia virus strain 745A; the clone DS19 is described in the literature to be DMSO sensitive, resulting in erythroid differentiation |
| Reference(s) ： | 14545, 14816, 14965 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round, single or in small clusters, growing in suspension, about 5% giant cells; image; image; image |
| Medium ： | 80-90% Dulbeccos MEM + 10-20% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 5-10 x 104 cells/ml; split saturated culture 1:8 to 1:10 every 3 days; maintain at about 0.05-0.5 x 106 cells/ml; during continuous culture, the cell density should not be higher than 0.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 20 hours |
| Harvest ： | maximal density at about 0.8 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Cytogenetics ： | murine hypertetraploid karyotype |
| Viruses ： | PCR: SMRV - |
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| 产品名称： | MEL-745A cl. DS19 |
|---|---|
| 商品货号： | TS259757 |
| <strong>Cell line </strong>： | MEL-745A cl. DS19 |
| DSMZ no. ： | TS259757 |
| Species ： | confirmed as mouse by species PCR and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | erythroleukemia |
| Origin ： | Mouse erythroleukemia (MEL) cells were transformed by Friend leukemia virus strain 745A; the clone DS19 is described in the literature to be DMSO sensitive, resulting in erythroid differentiation |
| Reference(s) ： | 14545, 14816, 14965 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round, single or in small clusters, growing in suspension, about 5% giant cells; image; image; image |
| Medium ： | 80-90% Dulbeccos MEM + 10-20% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 5-10 x 104 cells/ml; split saturated culture 1:8 to 1:10 every 3 days; maintain at about 0.05-0.5 x 106 cells/ml; during continuous culture, the cell density should not be higher than 0.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 20 hours |
| Harvest ： | maximal density at about 0.8 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Cytogenetics ： | murine hypertetraploid karyotype |
| Viruses ： | PCR: SMRV - |
