RS4;11
RS4;11
规格:
货期:
编号:TS259763
品牌:Testobio
| 产品名称： | RS4;11 |
|---|---|
| 商品货号： | TS259763 |
| <strong>Cell line </strong>： | RS4;11 |
| DSMZ no. ： | TS259763 |
| Species ： | originally confirmed as human by cytogenetics |
| Cell type ： | B cell precursor leukemia |
| Origin ： | established from the bone marrow of a 32-year-old woman with acute lymphoblastic leukemia (ALL L2) in first relapse |
| Reference(s) ： | 14493, 14988, 15227, 15228 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, relatively small, round cells growing in suspension; image ; image |
| Medium ： | 90% alpha-MEM (with ribo- and deoxyribonucleosides) + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.3-0.5 x 106 cells/ml; maintain at about 0.5-1.0 x 106 cells/ml; split saturated culture 1:3 to 1:6 every 3-4 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | maximal density of about 4.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 -, CD34 -, CD38 +, CD80 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype - 47/48<2n>X/XX, +8, +18, t(4;11)(q21;q23), i(7q) - sideline without +8/18 - carries KMT2A-AFF1 (MLL-AFF1; MLL-AF4) fusion gene - resembles published karyotype |
| Molec. Genetics ： | expression of fusion gene KMT2A-AFF1 (MLL-AFF1; MLL-AF4) e10-e4 confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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周雪
13372109301
| 产品名称： | RS4;11 |
|---|---|
| 商品货号： | TS259763 |
| <strong>Cell line </strong>： | RS4;11 |
| DSMZ no. ： | TS259763 |
| Species ： | originally confirmed as human by cytogenetics |
| Cell type ： | B cell precursor leukemia |
| Origin ： | established from the bone marrow of a 32-year-old woman with acute lymphoblastic leukemia (ALL L2) in first relapse |
| Reference(s) ： | 14493, 14988, 15227, 15228 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, relatively small, round cells growing in suspension; image ; image |
| Medium ： | 90% alpha-MEM (with ribo- and deoxyribonucleosides) + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.3-0.5 x 106 cells/ml; maintain at about 0.5-1.0 x 106 cells/ml; split saturated culture 1:3 to 1:6 every 3-4 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | maximal density of about 4.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 -, CD34 -, CD38 +, CD80 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype - 47/48<2n>X/XX, +8, +18, t(4;11)(q21;q23), i(7q) - sideline without +8/18 - carries KMT2A-AFF1 (MLL-AFF1; MLL-AF4) fusion gene - resembles published karyotype |
| Molec. Genetics ： | expression of fusion gene KMT2A-AFF1 (MLL-AFF1; MLL-AF4) e10-e4 confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
