TC-71
TC-71
规格:
货期:
编号:TS259769
品牌:Testobio
| 产品名称： | TC-71 |
|---|---|
| 商品货号： | TS259769 |
| <strong>Cell line </strong>： | TC-71 |
| DSMZ no. ： | TS259769 |
| Species ： | originally confirmed as human by cytogenetics |
| Cell type ： | Ewings sarcoma |
| Origin ： | established from the tumor of a 22-year-old man with metastatic Ewings sarcoma that arose in the humerus; cell line was derived in 1981 from a biopsy of recurrent tumor at the primary site |
| Reference(s) ： | 14948, 15058 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | loosely adherent cells growing in monolayers; image |
| Medium ： | 90% Iscoves MDM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:4 to 1:10 every 2-4 days; trypsin/EDTA is not necessary, cells can be detached by tapping the flask |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 24 hours |
| Harvest ： | cell harvest of about 15 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypertriploid karyotype with 4% polyploidy - 70-80<3n>XX, -Y, +1, +2, -3, +6, +8, -9, +11, -17, +21, +2-11 mar, add(3)(q29), add(6)(q25)x2-3, del(8)(p22), add(8)(p22), del(9(p24), t(11;22)(q24;q12)x2, i(13q)x1-2, add(14)(p11) - sideline with der(2)t(2;3)(p23;p11), add(11)(p15)x2 - carries two copies of t(11;22) effecting EWS-FLI-1 fusion, yielding type 1 (shorter) product - broadly resembles published karyotype |
| Molec. Genetics ： | expression of fusion gene EWS-FLI (type 1) confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | TC-71 |
|---|---|
| 商品货号： | TS259769 |
| <strong>Cell line </strong>： | TC-71 |
| DSMZ no. ： | TS259769 |
| Species ： | originally confirmed as human by cytogenetics |
| Cell type ： | Ewings sarcoma |
| Origin ： | established from the tumor of a 22-year-old man with metastatic Ewings sarcoma that arose in the humerus; cell line was derived in 1981 from a biopsy of recurrent tumor at the primary site |
| Reference(s) ： | 14948, 15058 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | loosely adherent cells growing in monolayers; image |
| Medium ： | 90% Iscoves MDM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:4 to 1:10 every 2-4 days; trypsin/EDTA is not necessary, cells can be detached by tapping the flask |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 24 hours |
| Harvest ： | cell harvest of about 15 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypertriploid karyotype with 4% polyploidy - 70-80<3n>XX, -Y, +1, +2, -3, +6, +8, -9, +11, -17, +21, +2-11 mar, add(3)(q29), add(6)(q25)x2-3, del(8)(p22), add(8)(p22), del(9(p24), t(11;22)(q24;q12)x2, i(13q)x1-2, add(14)(p11) - sideline with der(2)t(2;3)(p23;p11), add(11)(p15)x2 - carries two copies of t(11;22) effecting EWS-FLI-1 fusion, yielding type 1 (shorter) product - broadly resembles published karyotype |
| Molec. Genetics ： | expression of fusion gene EWS-FLI (type 1) confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
