CAL-29
CAL-29
规格:
货期:
编号:TS259770
品牌:Testobio
| 产品名称： | CAL-29 |
|---|---|
| 商品货号： | TS259770 |
| <strong>Cell line </strong>： | CAL-29 |
| DSMZ no. ： | TS259770 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | urinary bladder transitional cell carcinoma |
| Origin ： | established from the primary lesion of a fatally invasive, metastatic transitional cell carcinoma of the bladder (grade IV, stage T2) from an 80-year-old woman before treatment |
| Reference(s) ： | 14423 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells, some with spindle-like extensions growing adherently in monolayers; image; image ; image |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:4 every 3-6 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | maximal density of ca. 6.0 x 106 cells/80 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypertriploid karyotype with 8% polyploidy - 71(63-74)<3n>XX/XXX, -4, +9, -11, -13, -17, +20, +3mar, +1-6 dmin, i(3q), add(7)(q22)x1-2, del(7)(q32)x2, del(9)(p21), del(10)(p13), add(12)(p1?3), add(19)(p13) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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周雪
13372109301
| 产品名称： | CAL-29 |
|---|---|
| 商品货号： | TS259770 |
| <strong>Cell line </strong>： | CAL-29 |
| DSMZ no. ： | TS259770 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | urinary bladder transitional cell carcinoma |
| Origin ： | established from the primary lesion of a fatally invasive, metastatic transitional cell carcinoma of the bladder (grade IV, stage T2) from an 80-year-old woman before treatment |
| Reference(s) ： | 14423 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells, some with spindle-like extensions growing adherently in monolayers; image; image ; image |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:4 every 3-6 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | maximal density of ca. 6.0 x 106 cells/80 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypertriploid karyotype with 8% polyploidy - 71(63-74)<3n>XX/XXX, -4, +9, -11, -13, -17, +20, +3mar, +1-6 dmin, i(3q), add(7)(q22)x1-2, del(7)(q32)x2, del(9)(p21), del(10)(p13), add(12)(p1?3), add(19)(p13) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
