CL-34
CL-34
规格:
货期:
编号:TS259778
品牌:Testobio
| 产品名称： | CL-34 |
|---|---|
| 商品货号： | TS259778 |
| <strong>Cell line </strong>： | CL-34 |
| DSMZ no. ： | TS259778 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | colon carcinoma |
| Origin ： | established from a woman with a moderately differentiated primary adenocarcinoma of the right colon (TNM stage 2) |
| Reference(s) ： | 14528 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like cells growing in islets and as monolayer; cells do not grow to complete confluency; image; image |
| Medium ： | 80% mixture of Dulbeccos MEM + Hams F12 at 1:1 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 4 x 106 cells/80 cm2; split semi-confluent culture 1:4 to 1:6 once or twice a week using trypsin/EDTA for 10 min; after thawing the use of collagen-coated flasks for the first week may be of advantage |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 70 hours |
| Harvest ： | cell harvest of ca. 15 x 106 cells in a 175 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypodiploid karyotype with 16%polyploidy - 45(42-52)<2n>X/XX, -6 - sdl with +3, del(3)(q21) - near-normal "unstable" karyotype with many inconsistent structural and numerical changes |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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周雪
13372109301
| 产品名称： | CL-34 |
|---|---|
| 商品货号： | TS259778 |
| <strong>Cell line </strong>： | CL-34 |
| DSMZ no. ： | TS259778 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | colon carcinoma |
| Origin ： | established from a woman with a moderately differentiated primary adenocarcinoma of the right colon (TNM stage 2) |
| Reference(s) ： | 14528 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like cells growing in islets and as monolayer; cells do not grow to complete confluency; image; image |
| Medium ： | 80% mixture of Dulbeccos MEM + Hams F12 at 1:1 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 4 x 106 cells/80 cm2; split semi-confluent culture 1:4 to 1:6 once or twice a week using trypsin/EDTA for 10 min; after thawing the use of collagen-coated flasks for the first week may be of advantage |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 70 hours |
| Harvest ： | cell harvest of ca. 15 x 106 cells in a 175 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypodiploid karyotype with 16%polyploidy - 45(42-52)<2n>X/XX, -6 - sdl with +3, del(3)(q21) - near-normal "unstable" karyotype with many inconsistent structural and numerical changes |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
