TALL-1
TALL-1
规格:
货期:
编号:TS259781
品牌:Testobio
| 产品名称： | TALL-1 |
|---|---|
| 商品货号： | TS259781 |
| <strong>Cell line </strong>： | TALL-1 |
| DSMZ no. ： | TS259781 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | T cell leukemia |
| Origin ： | established from the bone marrow of a 28-year-old man who developed the terminal leukemic phase of lymphosarcoma in 1976 |
| Reference(s) ： | 14772 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single cells growing in suspension; image; image |
| Medium ： | 85% RPMI 1640 + 15% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-2.0 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 3-4 days; maximal density of ca. 2.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-70 hours |
| Harvest ： | cell harvest of about 2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture, PCR assays |
| Immunology ： | CD2 +, CD3 +, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta (+), TCRgamma/delta -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypertetraploid karyotype - 90-102<4n>XXY/XXYY, -3, -9, -12, +13, +14, +14, -15, +20, +20, +21, +21, +2-4mar, der(X;1)(p10;p10)/add(1)(q11), add(1)(p11), add(5)(p1?5), del(2)(p22p24), del(12)(q24.1), dup(21)(q11qter)x3-4 - resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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联系我们CONTACT US
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0574-87917803
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testobio@163.com
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浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
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联系方式
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | TALL-1 |
|---|---|
| 商品货号： | TS259781 |
| <strong>Cell line </strong>： | TALL-1 |
| DSMZ no. ： | TS259781 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | T cell leukemia |
| Origin ： | established from the bone marrow of a 28-year-old man who developed the terminal leukemic phase of lymphosarcoma in 1976 |
| Reference(s) ： | 14772 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single cells growing in suspension; image; image |
| Medium ： | 85% RPMI 1640 + 15% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-2.0 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 3-4 days; maximal density of ca. 2.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-70 hours |
| Harvest ： | cell harvest of about 2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture, PCR assays |
| Immunology ： | CD2 +, CD3 +, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta (+), TCRgamma/delta -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypertetraploid karyotype - 90-102<4n>XXY/XXYY, -3, -9, -12, +13, +14, +14, -15, +20, +20, +21, +21, +2-4mar, der(X;1)(p10;p10)/add(1)(q11), add(1)(p11), add(5)(p1?5), del(2)(p22p24), del(12)(q24.1), dup(21)(q11qter)x3-4 - resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
