KASUMI-2
KASUMI-2
规格:
货期:
编号:TS259795
品牌:Testobio
| 产品名称： | KASUMI-2 |
|---|---|
| 商品货号： | TS259795 |
| <strong>Cell line </strong>： | KASUMI-2 |
| DSMZ no. ： | TS259795 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | B cell precursor leukemia |
| Origin ： | established from the bone marrow of a 15-year-old boy with acute lymphoblastic leukemia (ALL FAB L2) in 1990 at diagnosis |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small round cells growing singly or in small clumps in suspension, some loosely adherent; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1.5 x 106 cells/ml; start culture in 24-well-plates in the presence of 20% FBS (for the first 1-2 weeks); split saturated culture 1:2 to 1:6 every 2-3 days; maintain at ca. 0.5-2.0 x 106 cells/ml; maximal density of ca. 7 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | cell harvest of ca. 7 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 +, CD19 +, CD20 +, CD34 -, CD37 -, CD38 +, CD80 -, HLA-DR +, sm/cyIgG -, smIgM -, cyIgM +, sm/cykappa -, sm/cylamda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypodiploid karyotype with 3% polyploidy - 45(44-46)<2n>X, -Y, del(2)(p23.2/p21p23.1), t(3;14)(q27-28;q24), del(6)(q21), i(7q), del(13)(q1?3q1?4), der(19)t(1;19)(q23;p13) - sideline with idem 46, XY - carries der(19) partner of t(1;19) with TCF3 (E2A) rearrangement confirmed by FISH |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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KASUMI-2
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0574-87917803
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testobio@163.com
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浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
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联系方式
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | KASUMI-2 |
|---|---|
| 商品货号： | TS259795 |
| <strong>Cell line </strong>： | KASUMI-2 |
| DSMZ no. ： | TS259795 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | B cell precursor leukemia |
| Origin ： | established from the bone marrow of a 15-year-old boy with acute lymphoblastic leukemia (ALL FAB L2) in 1990 at diagnosis |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small round cells growing singly or in small clumps in suspension, some loosely adherent; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1.5 x 106 cells/ml; start culture in 24-well-plates in the presence of 20% FBS (for the first 1-2 weeks); split saturated culture 1:2 to 1:6 every 2-3 days; maintain at ca. 0.5-2.0 x 106 cells/ml; maximal density of ca. 7 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | cell harvest of ca. 7 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 +, CD19 +, CD20 +, CD34 -, CD37 -, CD38 +, CD80 -, HLA-DR +, sm/cyIgG -, smIgM -, cyIgM +, sm/cykappa -, sm/cylamda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypodiploid karyotype with 3% polyploidy - 45(44-46)<2n>X, -Y, del(2)(p23.2/p21p23.1), t(3;14)(q27-28;q24), del(6)(q21), i(7q), del(13)(q1?3q1?4), der(19)t(1;19)(q23;p13) - sideline with idem 46, XY - carries der(19) partner of t(1;19) with TCF3 (E2A) rearrangement confirmed by FISH |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
