DND-41
DND-41
规格:
货期:
编号:TS259797
品牌:Testobio
| 产品名称： | DND-41 |
|---|---|
| 商品货号： | TS259797 |
| <strong>Cell line </strong>： | DND-41 |
| DSMZ no. ： | TS259797 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | T cell leukemia |
| Origin ： | established from the peripheral blood of 13-year-old boy with T-acute lymphoblastic leukemia (T-ALL; type III cortical) in 1977; described in the literature to carry CDKN2B (p15INK4B) and CDKN2A (p16INK4A) deletions and TP53 (p53) mutation. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14473, 14489, 15217, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly or, occasionally, in clumps; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-1.0 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-4 days; maintain at ca. 0.3-1.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35 hours |
| Harvest ： | cell harvest of ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 +, CD6 -, CD7 +, CD8 -, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded near-tetraploid karyotype with 12% polyploidy - 92(86-98)<4n>XXYY, -9, -15, +20, +mar - resembles published karyotype |
| Molec. Genetics ： | expression of TLX3 confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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周雪
13372109301
| 产品名称： | DND-41 |
|---|---|
| 商品货号： | TS259797 |
| <strong>Cell line </strong>： | DND-41 |
| DSMZ no. ： | TS259797 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | T cell leukemia |
| Origin ： | established from the peripheral blood of 13-year-old boy with T-acute lymphoblastic leukemia (T-ALL; type III cortical) in 1977; described in the literature to carry CDKN2B (p15INK4B) and CDKN2A (p16INK4A) deletions and TP53 (p53) mutation. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14473, 14489, 15217, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly or, occasionally, in clumps; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-1.0 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-4 days; maintain at ca. 0.3-1.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35 hours |
| Harvest ： | cell harvest of ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 +, CD6 -, CD7 +, CD8 -, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded near-tetraploid karyotype with 12% polyploidy - 92(86-98)<4n>XXYY, -9, -15, +20, +mar - resembles published karyotype |
| Molec. Genetics ： | expression of TLX3 confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
