OCI-LY19
OCI-LY19
规格:
货期:
编号:TS259799
品牌:Testobio
| 产品名称： | OCI-LY19 |
|---|---|
| 商品货号： | TS259799 |
| <strong>Cell line </strong>： | OCI-LY19 |
| DSMZ no. ： | TS259799 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | B cell lymphoma |
| Origin ： | established from the bone marrow of a 27-year-old woman with B-cell non-Hodgkin lymphoma (B-NHL) (diffuse large cell lymphoma, DLCL, stage 4B, at relapse) in 1987. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14427, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round cells growing in suspension; image; image |
| Medium ： | 80-90% alpha-MEM (with ribo- and deoxyribonucleosides) + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; split saturated culture 1:4 to 1:6 every 3-5 days; maintain at ca. 0.5-2.0 x 106 cells/ml; one day after thawing the viability may drop down to 50%, but cells recover within one week |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | cell harvest of ca. 3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 (+), CD37 -, CD38 +, cyCD79a +, HLA-DR +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 4% polyploidy - 48(46-52)<2n>X, -X, +6, +6, +8, t(4;8)(q3?2;q?24), del(6)(q15)x2, r(8)(??), t(14;18)(q32;q21), add(18)(q23) - carries t(14;18) effecting IGH-BCL2 juxtaposition |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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联系我们CONTACT US
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0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | OCI-LY19 |
|---|---|
| 商品货号： | TS259799 |
| <strong>Cell line </strong>： | OCI-LY19 |
| DSMZ no. ： | TS259799 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | B cell lymphoma |
| Origin ： | established from the bone marrow of a 27-year-old woman with B-cell non-Hodgkin lymphoma (B-NHL) (diffuse large cell lymphoma, DLCL, stage 4B, at relapse) in 1987. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14427, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round cells growing in suspension; image; image |
| Medium ： | 80-90% alpha-MEM (with ribo- and deoxyribonucleosides) + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; split saturated culture 1:4 to 1:6 every 3-5 days; maintain at ca. 0.5-2.0 x 106 cells/ml; one day after thawing the viability may drop down to 50%, but cells recover within one week |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | cell harvest of ca. 3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 (+), CD37 -, CD38 +, cyCD79a +, HLA-DR +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 4% polyploidy - 48(46-52)<2n>X, -X, +6, +6, +8, t(4;8)(q3?2;q?24), del(6)(q15)x2, r(8)(??), t(14;18)(q32;q21), add(18)(q23) - carries t(14;18) effecting IGH-BCL2 juxtaposition |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
