| 产品名称: | AMO-1 |
|---|---|
| 商品货号: | TS259818 |
| <strong>Cell line </strong>: | AMO-1 |
| DSMZ no. : | TS259818 |
| Species : | human (Homo sapiens) |
| Cell type : | plasmacytoma |
| Origin : | established from the ascitic fluid of a 64-year-old woman with plasmacytoma (IgAkappa) of the duodenum two months following resection of the tumor mass (additional therapy not specified) in 1984; plasmacytoma is related to multiple myeloma |
| Reference(s) : | 14486, 14956 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | round cells growing singly in suspension; image; image; image |
| Medium : | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture : | seed out at ca. 0.8 x 106 cells/ml; maintain at ca. 0.5-1.2 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-4 days; minimum density at ca. 0.5 x 106 cells/ml |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 70-90 hours |
| Harvest : | maximum cell density of ca. 1.8 x 106 cells/ml |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture, PCR assays |
| Immunology : | CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD38 +, cyCD79a+, CD80 -, CD138 +, HLA-DR +, cy IgG -, cy IgM -, cy kappa +, cy lambda -; image |
| Fingerprint : | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| 产品名称: | AMO-1 |
|---|---|
| 商品货号: | TS259818 |
| <strong>Cell line </strong>: | AMO-1 |
| DSMZ no. : | TS259818 |
| Species : | human (Homo sapiens) |
| Cell type : | plasmacytoma |
| Origin : | established from the ascitic fluid of a 64-year-old woman with plasmacytoma (IgAkappa) of the duodenum two months following resection of the tumor mass (additional therapy not specified) in 1984; plasmacytoma is related to multiple myeloma |
| Reference(s) : | 14486, 14956 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | round cells growing singly in suspension; image; image; image |
| Medium : | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture : | seed out at ca. 0.8 x 106 cells/ml; maintain at ca. 0.5-1.2 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-4 days; minimum density at ca. 0.5 x 106 cells/ml |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 70-90 hours |
| Harvest : | maximum cell density of ca. 1.8 x 106 cells/ml |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture, PCR assays |
| Immunology : | CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD38 +, cyCD79a+, CD80 -, CD138 +, HLA-DR +, cy IgG -, cy IgM -, cy kappa +, cy lambda -; image |
| Fingerprint : | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |