DB
DB
规格:
货期:
编号:TS259823
品牌:Testobio
| 产品名称： | DB |
|---|---|
| 商品货号： | TS259823 |
| <strong>Cell line </strong>： | DB |
| DSMZ no. ： | TS259823 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | B cell lymphoma |
| Origin ： | established from ascites of a 45-year-old Caucasian man with diffuse large cell lymphoma; cell line carries EZH2 Y641N mutation; assigned to GCB-like lymphoma subtype (germinal center B-cell) |
| Reference(s) ： | 14375, 16553, 17354 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round single cells in suspension (partly in clusters or clumps) |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | split saturated culture 1:2 to 1:3 every 2-3 days; seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-2.0 x 106 cells/ml; minimum density at ca. 0.4 x 106 cells/ml; after thawing cultivate in 6-well-plates during the first 2 weeks |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 -, CD10 +, CD13 -, CD20 +, CD37 +, HLA-DR -, cyIgG +, cy IgM -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid karyotype - 80(79-81)<3n>XXXYY, -3, +7, +7, +8, -9, +11, -13, +16, +18, +20, +20, +20, +21, +22, +mar, der(4)t(X;4)(q11;p13), add(5)(q11), t(10;20)(q24;p11), ?inv(13)(q21.2q32), t(14;18)(q32;q21), del(16)(p13.2), der(18)(t(14;18)(q32;q21) - carries t(14;18) associated with B-cell lymphoma |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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朱静
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李晴
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李晶晶
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周雪
13372109301
| 产品名称： | DB |
|---|---|
| 商品货号： | TS259823 |
| <strong>Cell line </strong>： | DB |
| DSMZ no. ： | TS259823 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | B cell lymphoma |
| Origin ： | established from ascites of a 45-year-old Caucasian man with diffuse large cell lymphoma; cell line carries EZH2 Y641N mutation; assigned to GCB-like lymphoma subtype (germinal center B-cell) |
| Reference(s) ： | 14375, 16553, 17354 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round single cells in suspension (partly in clusters or clumps) |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | split saturated culture 1:2 to 1:3 every 2-3 days; seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-2.0 x 106 cells/ml; minimum density at ca. 0.4 x 106 cells/ml; after thawing cultivate in 6-well-plates during the first 2 weeks |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 -, CD10 +, CD13 -, CD20 +, CD37 +, HLA-DR -, cyIgG +, cy IgM -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid karyotype - 80(79-81)<3n>XXXYY, -3, +7, +7, +8, -9, +11, -13, +16, +18, +20, +20, +20, +21, +22, +mar, der(4)t(X;4)(q11;p13), add(5)(q11), t(10;20)(q24;p11), ?inv(13)(q21.2q32), t(14;18)(q32;q21), del(16)(p13.2), der(18)(t(14;18)(q32;q21) - carries t(14;18) associated with B-cell lymphoma |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
