H-1963
H-1963
规格:
货期:
编号:TS259827
品牌:Testobio
| 产品名称： | H-1963 |
|---|---|
| 商品货号： | TS259827 |
| <strong>Cell line </strong>： | H-1963 |
| DSMZ no. ： | TS259827 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | small cell lung carcinoma |
| Origin ： | derived from the lung of a 56-year-old black man with small cell lung carcinoma prior to treatment in 1988 |
| Reference(s) ： | 14849 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | cells grow in clumps in suspension |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | split culture 1:2 every 3 days; seed out at ca. 0.5 x 106 cells/ml; cell number can only be estimated due to the formation of large aggregates |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 3-4 days |
| Harvest ： | cell harvest of ca. 1-2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human bimodal hypodiploid/hypotetraploid aryotype with 3% polyploidy - 44(42-48)<2n>X, -Y, -5, -10, -13, +18, -19, +2mar, add(1)(q32), ins(2;hsr)(p21-23;?), add(3)(p11), del(4)(q24-26), del(10)(p13-14), add(18)(p11)x2, del(20)(q12), der(22)(t1;22)(q12;q13), dmin present in some cells |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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周雪
13372109301
| 产品名称： | H-1963 |
|---|---|
| 商品货号： | TS259827 |
| <strong>Cell line </strong>： | H-1963 |
| DSMZ no. ： | TS259827 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | small cell lung carcinoma |
| Origin ： | derived from the lung of a 56-year-old black man with small cell lung carcinoma prior to treatment in 1988 |
| Reference(s) ： | 14849 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | cells grow in clumps in suspension |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | split culture 1:2 every 3 days; seed out at ca. 0.5 x 106 cells/ml; cell number can only be estimated due to the formation of large aggregates |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 3-4 days |
| Harvest ： | cell harvest of ca. 1-2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human bimodal hypodiploid/hypotetraploid aryotype with 3% polyploidy - 44(42-48)<2n>X, -Y, -5, -10, -13, +18, -19, +2mar, add(1)(q32), ins(2;hsr)(p21-23;?), add(3)(p11), del(4)(q24-26), del(10)(p13-14), add(18)(p11)x2, del(20)(q12), der(22)(t1;22)(q12;q13), dmin present in some cells |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
