SEM
SEM
规格:
货期:
编号:TS259830
品牌:Testobio
| 产品名称： | SEM |
|---|---|
| 商品货号： | TS259830 |
| <strong>Cell line </strong>： | SEM |
| DSMZ no. ： | TS259830 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | B cell precursor leukemia |
| Origin ： | established from the peripheral blood of a 5-year-old girl at relapse of acute lymphoblastic leukemia (ALL) in 1990; reported to carry the t(4;11) KMT2A-AFF1 (MLL-AFF1; MLL-AF4). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14493, 14887, 14577, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polygonal single rather small cells in suspension; image; image |
| Medium ： | 90% Iscoves MDM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-2.0 x 106 cells/ml; split saturated culture 1:2 to 1:6 every 2-3 days; maximum density at ca. 3-4 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | cell harvest of ca. 3-4 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3-, CD4-, CD11b-, CD13+, CD14-, CD15+, CD19+, CD33+, CD34-, cyCD68-, HLA-DR+, sm/cyIgG-, sm/cyIgM-, sm/cykappa-, sm/cylamda-; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypodiploid karyotype with 1.5% polyploidy - 45(40-46)<2n>XX, -13, t(4;11)(q21;q23), del(7)(p14) - carries t(4;11) with breakpoints at AFF1 (AF4) and KMT2A (MLL) - matches published karyotype |
| Molec. Genetics ： | expression of fusion gene KMT2A-AFF1 (MLL-AFF1; MLL-AF4) confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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朱静
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周雪
13372109301
| 产品名称： | SEM |
|---|---|
| 商品货号： | TS259830 |
| <strong>Cell line </strong>： | SEM |
| DSMZ no. ： | TS259830 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | B cell precursor leukemia |
| Origin ： | established from the peripheral blood of a 5-year-old girl at relapse of acute lymphoblastic leukemia (ALL) in 1990; reported to carry the t(4;11) KMT2A-AFF1 (MLL-AFF1; MLL-AF4). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14493, 14887, 14577, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polygonal single rather small cells in suspension; image; image |
| Medium ： | 90% Iscoves MDM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-2.0 x 106 cells/ml; split saturated culture 1:2 to 1:6 every 2-3 days; maximum density at ca. 3-4 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | cell harvest of ca. 3-4 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3-, CD4-, CD11b-, CD13+, CD14-, CD15+, CD19+, CD33+, CD34-, cyCD68-, HLA-DR+, sm/cyIgG-, sm/cyIgM-, sm/cykappa-, sm/cylamda-; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypodiploid karyotype with 1.5% polyploidy - 45(40-46)<2n>XX, -13, t(4;11)(q21;q23), del(7)(p14) - carries t(4;11) with breakpoints at AFF1 (AF4) and KMT2A (MLL) - matches published karyotype |
| Molec. Genetics ： | expression of fusion gene KMT2A-AFF1 (MLL-AFF1; MLL-AF4) confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
