RCH-ACV
RCH-ACV
规格:
货期:
编号:TS259836
品牌:Testobio
| 产品名称： | RCH-ACV |
|---|---|
| 商品货号： | TS259836 |
| <strong>Cell line </strong>： | RCH-ACV |
| DSMZ no. ： | TS259836 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | B cell precursor leukemia |
| Origin ： | established from bone marrow cells taken at relapse of common acute lymphoblastic leukemia (cALL), seven months after diagnosis, from an 8-year-old girl treated with combination chemotherapy |
| Reference(s) ： | 14634, 14661 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small round cells growing singly or in small clumps in suspension; occasional adherent cells present; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/ml; split saturated culture 1:2 to 1:10 every 2-6 days; maintain at ca. 0.5-2.5 x 106 cells/ml; easy to culture, tolerates low cell densities |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-50 hours |
| Harvest ： | cell harvest of ca. 4 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD13 -, CD19 +, CD37 -, cyCD79a +, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, smIgM -, cyIgM +, sm/cykappa -, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hyperdiploid karyotype with 2% polyploidy - 43-50<2n>XX, +8, t(1;19)(q23;p13.3) - sideline with idem, +16, +der(19)t(1;19) - carries t(1;19) effecting TCF3-PBX1 (E2A-PBX) fusion - matches published karyotype |
| Molec. Genetics ： | expression of fusion gene TCF3-PBX1 (E2A-PBX) confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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联系我们CONTACT US
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0574-87917803
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testobio@163.com
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浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
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联系方式
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | RCH-ACV |
|---|---|
| 商品货号： | TS259836 |
| <strong>Cell line </strong>： | RCH-ACV |
| DSMZ no. ： | TS259836 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | B cell precursor leukemia |
| Origin ： | established from bone marrow cells taken at relapse of common acute lymphoblastic leukemia (cALL), seven months after diagnosis, from an 8-year-old girl treated with combination chemotherapy |
| Reference(s) ： | 14634, 14661 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small round cells growing singly or in small clumps in suspension; occasional adherent cells present; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/ml; split saturated culture 1:2 to 1:10 every 2-6 days; maintain at ca. 0.5-2.5 x 106 cells/ml; easy to culture, tolerates low cell densities |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-50 hours |
| Harvest ： | cell harvest of ca. 4 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD13 -, CD19 +, CD37 -, cyCD79a +, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, smIgM -, cyIgM +, sm/cykappa -, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hyperdiploid karyotype with 2% polyploidy - 43-50<2n>XX, +8, t(1;19)(q23;p13.3) - sideline with idem, +16, +der(19)t(1;19) - carries t(1;19) effecting TCF3-PBX1 (E2A-PBX) fusion - matches published karyotype |
| Molec. Genetics ： | expression of fusion gene TCF3-PBX1 (E2A-PBX) confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
