C6
C6
规格:
货期:
编号:TS259838
品牌:Testobio
| 产品名称： | C6 |
|---|---|
| 商品货号： | TS259838 |
| <strong>Cell line </strong>： | C6 |
| DSMZ no. ： | TS259838 |
| Species ： | confirmed as rat by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type ： | glioma |
| Origin ： | the glial cell strain C6 was cloned from a rat glioma which was induced by N-nitrosomethylurea |
| Reference(s) ： | 14380 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent fibroblastoid cells forming spindles and growing in monolayers; image; image |
| Medium ： | 82.5% Hams F12 + 15% horse serum + 2.5% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | split confluent culture 1:20 every 2-3 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/175 cm2 flask |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 25-30 hours |
| Harvest ： | maximal density at ca. 2.0 x 107 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Cytogenetics ： | rat hyperdiploid karyotype with 4% polyploidy - 43(40-44)<2n> |
| Viruses ： | PCR: SMRV - |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | C6 |
|---|---|
| 商品货号： | TS259838 |
| <strong>Cell line </strong>： | C6 |
| DSMZ no. ： | TS259838 |
| Species ： | confirmed as rat by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type ： | glioma |
| Origin ： | the glial cell strain C6 was cloned from a rat glioma which was induced by N-nitrosomethylurea |
| Reference(s) ： | 14380 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent fibroblastoid cells forming spindles and growing in monolayers; image; image |
| Medium ： | 82.5% Hams F12 + 15% horse serum + 2.5% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | split confluent culture 1:20 every 2-3 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/175 cm2 flask |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 25-30 hours |
| Harvest ： | maximal density at ca. 2.0 x 107 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Cytogenetics ： | rat hyperdiploid karyotype with 4% polyploidy - 43(40-44)<2n> |
| Viruses ： | PCR: SMRV - |
