KMS-12-BM
KMS-12-BM
规格:
货期:
编号:TS259843
品牌:Testobio
| 产品名称： | KMS-12-BM |
|---|---|
| 商品货号： | TS259843 |
| <strong>Cell line </strong>： | KMS-12-BM |
| DSMZ no. ： | TS259843 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | multiple myeloma |
| Origin ： | established from the bone marrow of a 64-year-old woman with multiple myeloma in 1988; sister cell line of KMS-12-PE (DSM ACC 606). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14817, 14486, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | mostly single cells in suspension, 1-2% giant cells; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/m; split saturated culture 1:2 every 2-3 days; maintain at ca. 0.5-1.0 x 106 cells/ml; first week after thawing cultivate in 24-well-plate, initially cells grow slowly |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60 hours |
| Harvest ： | cell harvest of ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 -, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 -, CD138 +, HLA-DR -, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile (identical to that of sister cell line KMS-12-PE; ACC 606) |
| Cytogenetics ： | human flat-moded hypertriploid karyotype with 3% polyploidy - 69-79<3n>XX, -X, +1, +1, -4, +5, +6, +8, +9, -10, +13, +14, -15, -16, +17, -18, +19, +20, +21, -22, +4-5mar, der(1)t(1;4)(q11;q32), del(1)(p21)x2, der(1;8)(q10;q10)x2, add(4)(q32)x1-2, der(5;17)(p10;q10)x2, add(6)(q2?5), der(9)add(9)(p24)t(9;11)(q34;q13), der(9)t(9;11)(q34;q13), der(11)t(11;14)(q13;q32)x2-3, idic(?;13)(?;q32-33)x2, der(14)t(11;14)(q13;q32), der(17)t(11;17)(q21.2;q22)t(11;14)(q13;q32)x2, add(19)(q13), add(19)(q13) - related to published karyotype - carries semi-cryptic t(11;14) with IGH-CCND1 rearrangement |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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李晶晶
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周雪
13372109301
| 产品名称： | KMS-12-BM |
|---|---|
| 商品货号： | TS259843 |
| <strong>Cell line </strong>： | KMS-12-BM |
| DSMZ no. ： | TS259843 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | multiple myeloma |
| Origin ： | established from the bone marrow of a 64-year-old woman with multiple myeloma in 1988; sister cell line of KMS-12-PE (DSM ACC 606). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14817, 14486, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | mostly single cells in suspension, 1-2% giant cells; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/m; split saturated culture 1:2 every 2-3 days; maintain at ca. 0.5-1.0 x 106 cells/ml; first week after thawing cultivate in 24-well-plate, initially cells grow slowly |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60 hours |
| Harvest ： | cell harvest of ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 -, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 -, CD138 +, HLA-DR -, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile (identical to that of sister cell line KMS-12-PE; ACC 606) |
| Cytogenetics ： | human flat-moded hypertriploid karyotype with 3% polyploidy - 69-79<3n>XX, -X, +1, +1, -4, +5, +6, +8, +9, -10, +13, +14, -15, -16, +17, -18, +19, +20, +21, -22, +4-5mar, der(1)t(1;4)(q11;q32), del(1)(p21)x2, der(1;8)(q10;q10)x2, add(4)(q32)x1-2, der(5;17)(p10;q10)x2, add(6)(q2?5), der(9)add(9)(p24)t(9;11)(q34;q13), der(9)t(9;11)(q34;q13), der(11)t(11;14)(q13;q32)x2-3, idic(?;13)(?;q32-33)x2, der(14)t(11;14)(q13;q32), der(17)t(11;17)(q21.2;q22)t(11;14)(q13;q32)x2, add(19)(q13), add(19)(q13) - related to published karyotype - carries semi-cryptic t(11;14) with IGH-CCND1 rearrangement |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
