MOLM-13
MOLM-13
规格:
货期:
编号:TS259852
品牌:Testobio
| 产品名称： | MOLM-13 |
|---|---|
| 商品货号： | TS259852 |
| <strong>Cell line </strong>： | MOLM-13 |
| DSMZ no. ： | TS259852 |
| Species ： | confirmed as human by PCR speciation and cytogenetics |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established from the peripheral blood of a 20-year-old man with acute myeloid leukemia AML FAB M5a at relapse in 1995 after initial myelodysplastic syndromes (MDS, refractory anemia with excess of blasts, RAEB); carries internal tandem duplication of FLT3; the FLT3 protein is not expressed; cell line carries the CBL deltaExon8 mutant; sister cell line of MOLM-14 (ACC 777). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14869, 14743, 14493, 15073, 16558, 18138, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | most cells are round growing in suspension ; image ; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 1.0 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days; maintain at ca 0.4-2.0 x 106 cells/ml; viability may drop below 50% after thawing, but cells recover after 4-5 days (starting in a 12- or 24-well plate is recommended) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of ca. 2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 (+), CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +, HLA-DR -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a DNA profile identical to MOLM-14 |
| Cytogenetics ： | human hyperdiploid karyotype with 4% polyploidy - 51(48-52)<2n>XY, +8, +8, +8, +13, del(8)(p1?p2?), ins(11;9)(q23;p22p23) - resembles published karyotype - sideline with idem, +19 - carries occult insertion effecting KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) fusion |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
MOLM-13
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | MOLM-13 |
|---|---|
| 商品货号： | TS259852 |
| <strong>Cell line </strong>： | MOLM-13 |
| DSMZ no. ： | TS259852 |
| Species ： | confirmed as human by PCR speciation and cytogenetics |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established from the peripheral blood of a 20-year-old man with acute myeloid leukemia AML FAB M5a at relapse in 1995 after initial myelodysplastic syndromes (MDS, refractory anemia with excess of blasts, RAEB); carries internal tandem duplication of FLT3; the FLT3 protein is not expressed; cell line carries the CBL deltaExon8 mutant; sister cell line of MOLM-14 (ACC 777). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14869, 14743, 14493, 15073, 16558, 18138, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | most cells are round growing in suspension ; image ; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 1.0 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days; maintain at ca 0.4-2.0 x 106 cells/ml; viability may drop below 50% after thawing, but cells recover after 4-5 days (starting in a 12- or 24-well plate is recommended) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of ca. 2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 (+), CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +, HLA-DR -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a DNA profile identical to MOLM-14 |
| Cytogenetics ： | human hyperdiploid karyotype with 4% polyploidy - 51(48-52)<2n>XY, +8, +8, +8, +13, del(8)(p1?p2?), ins(11;9)(q23;p22p23) - resembles published karyotype - sideline with idem, +19 - carries occult insertion effecting KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) fusion |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
