HCC-827
HCC-827
规格:
货期:
编号:TS259874
品牌:Testobio
| 产品名称： | HCC-827 |
|---|---|
| 商品货号： | TS259874 |
| <strong>Cell line </strong>： | HCC-827 |
| DSMZ no. ： | TS259874 |
| Species ： | confirmed as human by cytogenetics and PCR species analysis |
| Cell type ： | non-small cell lung carcinoma |
| Origin ： | established from the lung of a 39-year-old woman with non-small cell lung cancer of the adenocarcinoma type; matched EBV+ B-lymphoblastoid cell line (B-LCL) is available (HCC-827BL) |
| Reference(s) ： | 14567, 15046 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | large epitheloid cells growing in monolayers; image; image |
| Medium ： | 80-85% RPMI 1640 + 15-20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2; split saturated culture 1:2 to 1:3 about twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | cell harvest of ca. 5-6 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotriploid karyotype with 6% polyploidy - 62(54-66)<3n>XXX/XXXX, -1, +7, +7, -9, -10, -15, -17, -18, -21, -22, +3 mar, +0-4 dmin, add(X)(p11), dup(2)(p1?p2?), add(3)(p11), add(6)(p21), add(7)(p12), add(7)(p22), add(7)(q21.2;HSR), add(21)(p11), der(21;22)(q10;q10), add(22)(p11) - 2 large submetacentric HSR markers present |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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周雪
13372109301
| 产品名称： | HCC-827 |
|---|---|
| 商品货号： | TS259874 |
| <strong>Cell line </strong>： | HCC-827 |
| DSMZ no. ： | TS259874 |
| Species ： | confirmed as human by cytogenetics and PCR species analysis |
| Cell type ： | non-small cell lung carcinoma |
| Origin ： | established from the lung of a 39-year-old woman with non-small cell lung cancer of the adenocarcinoma type; matched EBV+ B-lymphoblastoid cell line (B-LCL) is available (HCC-827BL) |
| Reference(s) ： | 14567, 15046 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | large epitheloid cells growing in monolayers; image; image |
| Medium ： | 80-85% RPMI 1640 + 15-20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2; split saturated culture 1:2 to 1:3 about twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | cell harvest of ca. 5-6 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotriploid karyotype with 6% polyploidy - 62(54-66)<3n>XXX/XXXX, -1, +7, +7, -9, -10, -15, -17, -18, -21, -22, +3 mar, +0-4 dmin, add(X)(p11), dup(2)(p1?p2?), add(3)(p11), add(6)(p21), add(7)(p12), add(7)(p22), add(7)(q21.2;HSR), add(21)(p11), der(21;22)(q10;q10), add(22)(p11) - 2 large submetacentric HSR markers present |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
