EN
EN
规格:
货期:
编号:TS259877
品牌:Testobio
| 产品名称： | EN |
|---|---|
| 商品货号： | TS259877 |
| <strong>Cell line </strong>： | EN |
| DSMZ no. ： | TS259877 |
| Species ： | confirmed as human by cytogenetics and species PCR |
| Cell type ： | endometrial carcinoma |
| Origin ： | established from the primary endometrial (cervical) carcinoma (stage IIIC) which was histologicall poorly differentiated from a 50-year-old Japanese woman prior to therapy |
| Reference(s) ： | 14630 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epitheloid cells growing in monolayers; image; image |
| Medium ： | 80% Dulbeccos MEM + 20% h.i. FBS |
| Subculture ： | split confluent culture 1:2 to 1:3 every 3-6 days using trypsin/EDTA; seed out 1.5 x 106 cells in 80 cm2; maintain at 0.1-0.2 x 105 cells/cm2; after recovery from N2 cell growth is slow (doubling time > 120 h) |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 50-80 hours |
| Harvest ： | cell harvest of ca. 1 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR revealed a unique STR DNA profile |
| Cytogenetics ： | human flat-moded near-tetraploid karyotype with 3% polyploidy - 79-97<4n>XXX/XXXX, -13, +2mar, 0-4 dm, der(1;7)(q10;q10)x2 - resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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周雪
13372109301
| 产品名称： | EN |
|---|---|
| 商品货号： | TS259877 |
| <strong>Cell line </strong>： | EN |
| DSMZ no. ： | TS259877 |
| Species ： | confirmed as human by cytogenetics and species PCR |
| Cell type ： | endometrial carcinoma |
| Origin ： | established from the primary endometrial (cervical) carcinoma (stage IIIC) which was histologicall poorly differentiated from a 50-year-old Japanese woman prior to therapy |
| Reference(s) ： | 14630 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epitheloid cells growing in monolayers; image; image |
| Medium ： | 80% Dulbeccos MEM + 20% h.i. FBS |
| Subculture ： | split confluent culture 1:2 to 1:3 every 3-6 days using trypsin/EDTA; seed out 1.5 x 106 cells in 80 cm2; maintain at 0.1-0.2 x 105 cells/cm2; after recovery from N2 cell growth is slow (doubling time > 120 h) |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 50-80 hours |
| Harvest ： | cell harvest of ca. 1 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR revealed a unique STR DNA profile |
| Cytogenetics ： | human flat-moded near-tetraploid karyotype with 3% polyploidy - 79-97<4n>XXX/XXXX, -13, +2mar, 0-4 dm, der(1;7)(q10;q10)x2 - resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
