SU-DHL-5
SU-DHL-5
规格:
货期:
编号:TS259880
品牌:Testobio
| 产品名称： | SU-DHL-5 |
|---|---|
| 商品货号： | TS259880 |
| <strong>Cell line </strong>： | SU-DHL-5 |
| DSMZ no. ： | TS259880 |
| Species ： | confirmed as human by cytogenetics and species PCR |
| Cell type ： | B cell lymphoma |
| Origin ： | established from a lymph node of a 17-year-old woman with B-cell non-Hodgkin lymphoma (B-NHL), described at the time as diffuse large cell, noncleaved cell type; assigned to GCB-like lymphoma subtype (germinal center B-cell) |
| Reference(s) ： | 14605, 15065, 14515, 17354, 14516 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small, round to polymorph cells growing mostly in clumps; image; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | initially seed out at ca. 0.5 x 106 cells/ml in a 24-well-plate (the viability may drop below 30% after thawing); later, maintain at ca. 0.1-1.0 x 106 cells/ml; split saturated culture 1:2 to 1:5 every 2-3 days; note: viability does usually not exceed 85% |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | cell harvest of ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD37 +, cyCD79a +, CD80 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 1.5% polyploidy - 47(41-48)<2n>XX, +12, del(6)(q13), del(12)(q13) - sideline with del(6)x2 - matches published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | SU-DHL-5 |
|---|---|
| 商品货号： | TS259880 |
| <strong>Cell line </strong>： | SU-DHL-5 |
| DSMZ no. ： | TS259880 |
| Species ： | confirmed as human by cytogenetics and species PCR |
| Cell type ： | B cell lymphoma |
| Origin ： | established from a lymph node of a 17-year-old woman with B-cell non-Hodgkin lymphoma (B-NHL), described at the time as diffuse large cell, noncleaved cell type; assigned to GCB-like lymphoma subtype (germinal center B-cell) |
| Reference(s) ： | 14605, 15065, 14515, 17354, 14516 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small, round to polymorph cells growing mostly in clumps; image; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | initially seed out at ca. 0.5 x 106 cells/ml in a 24-well-plate (the viability may drop below 30% after thawing); later, maintain at ca. 0.1-1.0 x 106 cells/ml; split saturated culture 1:2 to 1:5 every 2-3 days; note: viability does usually not exceed 85% |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | cell harvest of ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD37 +, cyCD79a +, CD80 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 1.5% polyploidy - 47(41-48)<2n>XX, +12, del(6)(q13), del(12)(q13) - sideline with del(6)x2 - matches published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
